Cancer Vaccine
Target Report

SampleAY

Cancer Typeunspecified

Compiled2026-09-28

This report ranks candidate vaccine targets from the patient's WES + RNA-seq.

Top candidate: STAG3 (missense, p.D893Y) — tier 1, CD8 0.715 / CD4 0.716, joint 1.012.

250+

Total quality candidates

250

SNV / indel candidates

candidate fusions

structural variants

Over 250 quality candidates were found; this report contains only the top 250 identified as most relevant to the patient's care.

Immunogenicity Scatter

How immunogenic each candidate is predicted to be for CD8 and CD4 T cells, plotted on shared axes.

Top-30 Candidates

The ranked candidate table with peptide windows, allele fractions, expression, and per-variant IGV read pileups (omitted from this public copy).

Joint Immunogenicity Scatter

This is a scatter plot of how immunogenic the patient's top candidate mutations are in 2 immune system pathways. The NEMo predicted probabilities are scaled against peptides assayed in vaccine trials — those that elicited an ELISPOT-positive CD8 or CD4 T-cell response and those that drove no response; together they anchor the score scale. The two marginal panels, the top strip (CD8 axis) and the right strip (CD4 axis), show the signed difference of validated-vaccine-peptide KDEs (f_immunogenic − f_{non-immunogenic}): colored regions are score ranges where ELISPOT-positive vax peptides concentrate. Each panel's zero crossing sets that axis's dashed line: the score above which the model finds more immunogenic than non-immunogenic vax peptides.

Together the two dashed lines split the plot into four quadrants:

Quadrant 1 (upper right): high on both axes, so the peptide is likely immunogenic to both CD4 (helper) and CD8 (cytotoxic) T cells.
Quadrant 2 (upper left): the peptide is predicted to be responsive for CD4 (helper) T cells.
Quadrant 3 (lower left): the peptide is not likely to be immunogenic to either T-cell subset.
Quadrant 4 (lower right): the peptide is predicted to be responsive for CD8 (cytotoxic) T cells.

Show

Marker size scales with RNA expression (TPM); fill scales with DNA AF.

Top-30 Vaccine Candidates

Each mutation-caused peptide carries a rank (#) reflecting its predicted treatment value. Ranked first by clinical confidence tier, then within a tier by joint CD8/CD4 immunogenicity. Toggleable display can show up to the top 250 candidate peptides, it is possible more than 250 potential targets exist.

Tier	#	Samples	Type	Gene	HGVSp	DNA AF	RNA AF	RNA_TPM	Best CD8 epitope	Best CD4 epitope	Vaccine peptide (25mer)	CD8 Probability	CD4 Probability	IGV
1	1	2	missense	STAG3	p.D893Y	0.237	0.140	4.4	YVFKHYNKF	AASYVFKHYNKFYND	YGVLEMDAASYVFKHYNKFYNDYGD	0.715	0.716	Snapshot omitted
1	2	2	missense	KLHL26	p.V126M	0.255	0.030	6.5	FAYSAEMTL	RGLRHIIDFAYSAEM	RGLRHIIDFAYSAEMTLDLDCVQDV	0.756	0.477	Snapshot omitted
1	3	2	stop_lost	PRKDC	p.4129Gext14	0.320	0.470	1846.0	SADRKHYIV	WEPWMGGLWESADRK	GRTWEGWEPWMGGLWESADRKHYIV	0.650	0.590	Snapshot omitted
1	4	2	missense	FOXL2	p.A234E	0.635	0.570	4.8	AEGPGSPGAAA	AAAAAAAAAAAAEGP	AAAAAAAAAAAAAEGPGSPGAAAVV	0.454	0.706	Snapshot omitted
1	5	2	missense	PKD1	p.R3130W	0.465	0.600	49.9	YEILVKTGWGW	TGWGWGSGTTAHVGI	FKYEILVKTGWGWGSGTTAHVGIML	0.507	0.654	Snapshot omitted
1	6	2	missense	CARF	p.D455N	0.248	0.250	5.1	KPNEVPERHNL	RKFVERELFKPNEVP	QLRKFVERELFKPNEVPERHNLSFF	0.598	0.533	Snapshot omitted
1	7	2	missense	FNDC1	p.R336H	0.272	0.200	6.7	TVYEFAVHI	PDTVYEFAVHISQGE	IENLIPDTVYEFAVHISQGERDGKW	0.561	0.545	Snapshot omitted
1	8	2	missense	MYH7B	p.A549D	0.198	0.460	3.5	ASDDSFRAKL	DDSFRAKLYDNHAGK	EECMFPKASDDSFRAKLYDNHAGKS	0.578	0.514	Snapshot omitted
1	9	2	missense	GALK2	p.G450D	0.280	0.600	45.4	KPGDGALVL	PEKQSLFATKPGDGA	LAPEKQSLFATKPGDGALVLLEA	0.760	0.120	Snapshot omitted
1	10	2	frameshift	BCAS1	p.V461Gfs*28	0.206		50.2	KEKKANQEPL	KSKEVESALQTGTST	STKEMLHLNPQKRNSKEKKANQEPL⚠	0.580	0.495	Snapshot omitted
1	11	2	missense	TRIM14	p.V68L	0.283	0.170	29.3	LALEAAVHL	HPVGLALEAAVHLQK	RGHPVGLALEAAVHLQKLSQECLK	0.668	0.354	Snapshot omitted
1	12	2	missense	DAGLB	p.V28G	0.454	0.150	15.2	FELVGRVLW	SDDLVFPGFFELVGR	ASDDLVFPGFFELVGRVLWWIGILT	0.536	0.481	Snapshot omitted
1	13	2	missense	GPR157	p.P314A	0.348	0.600	5.7	SPAGTPKAA	AGTPKAAAPSKPGES	PPTKSPAGTPKAAAPSKPGESQES	0.448	0.543	Snapshot omitted
1	14	2	missense	SLC39A10	p.M740I	0.239	0.570	17.2	SAIMAYIGM	VKQAIVYNLLSAIMA	MTVKQAIVYNLLSAIMAYIGMLIGT	0.443	0.533	Snapshot omitted
1	15	2	missense	HMCN1	p.V4127I	0.248	0.170	3.1	ILSSGSLQI	RAIVESIRQRILSSG	KDGRAIVESIRQRILSSGSLQIAFV	0.444	0.526	Snapshot omitted
1	16	2	missense	WWC1	p.D1064E	0.267	0.590	32.1	GELQTEKMM	KGELQTEKMMRAAAK	DRAEHKGELQTEKMMRAAAKDVHRL	0.441	0.525	Snapshot omitted
1	17	2	missense	SMARCA4	p.D1177Y	0.291	0.370	3386.1	SADTVIIFDSY	ADTVIIFDSYWNPHQ	LNLQSADTVIIFDSYWNPHQDLQA	0.516	0.444	Snapshot omitted
1	18	2	missense	TLN2	p.E2148Q	0.343	0.640	63.3	ALQATIECI	DEATRGTRALQATIE	KAVEDEATRGTRALQATIECIKQEL	0.395	0.524	Snapshot omitted
1	19	2	missense	PAN2	p.E602A	0.276		30.6	AASGKGNL	LGLILADSDAASGKG	EASALGLILADSDAASGKGNLARLI	0.310	0.565	Snapshot omitted
1	20	2	missense	SSTR4	p.L351I	0.218	1.000	1.4	PLDYYATAI	EPLDYYATAIKSKGG	GGAEEEPLDYYATAIKSKGGAGCM	0.383	0.499	Snapshot omitted
1	21	2	missense	HGF	p.C149F	0.427		17.0	SITKSGIKF	KSGIKFQPWSSMIPH	GTVSITKSGIKFQPWSSMIPHEHSF	0.312	0.536	Snapshot omitted
1	22	2	missense	WDFY1	p.V390I	0.236	0.270	40.1	GTDRIIKI	TDRIIKIWDMTPVVG	LMVTCGTDRIIKIWDMTPVVGCSLA	0.426	0.437	Snapshot omitted
1	23	2	missense	KMT2E	p.T897P	0.167	0.250	110.3	YAPPTHTDI	YAPPTHTDITPMDPS	TSTPTPSPYAPPTHTDITPMDPSFA	0.599	0.119	Snapshot omitted
1	24	2	missense	ZNF704	p.S127G	0.324	0.240	29.1	VPSSTSGSGY	GSWKEGGCVPSSTSG	GSWKEGGCVPSSTSGSGYWSWSAPS	0.288	0.523	Snapshot omitted
1	25	2	splice	CASP10	p.N240K	0.242	0.380	6.7	HAGSKGNRA	QNKHAGSKGNRATNG	PQESWQNKHAGSKGNRATNGAPSLV	0.106	0.534	Snapshot omitted
2	26	2	frameshift	PLXNA4	p.I1045Lfs*45	0.216		2.8	YVEDPTIVRL	EDPTIVRLSQNGALS	LVFQYVEDPTIVRLSQNGALSVETH	0.731	0.826	Snapshot omitted
2	27	2	missense	TTN	p.D20524Y	0.280		1.9	YLIQDLPRV	VYLIQDLPRVELQIK	GKVLVREKRVYLIQDLPRVELQIKE	0.915	0.483	Snapshot omitted
2	28	1	missense	PRRC2C	p.Y392N	0.202	0.430	141.0	VPNGKGPSF	PAQPSVAKVPNGKGP	SSQIPAQPSVAKVPNGKGPSFNQER	0.787	0.491	Snapshot omitted
2	29	1	missense	PLEKHG2	p.R1293W	0.176		12.0	YISQSLARW	RWQGPGGGAPAASRG	ASYISQSLARWQGPGGGAPAASRGS	0.303	0.810	Snapshot omitted
2	30	1	missense	ADPRHL1	p.R1805W	0.365	0.920	4.5	QERAWEQGW	WEQALTSGMAPRAWE	GAQERAWEQGWEQALTSGMAPRAWE	0.672	0.508	Snapshot omitted
2	31	2	missense	DNASE1L2	p.L11F	0.336		1.3	GPRALLAAF	PRALLAAFWALEAAG	MGGPRALLAAFWALEAAGTAALRIG	0.654	0.526	Snapshot omitted
2	32	2	missense	SLFN12L	p.C535G	0.576		3.0	GVMTKIFSL	KVGVMTKIFSLSPEG	AKIGGYTKKVGVMTKIFSLSPEGKT	0.622	0.546	Snapshot omitted
2	33	2	missense	GAPDHS	p.L174I	0.339		1.2	VVESTGVYI	YISIQAASDHISAGA	PYVVESTGVYISIQAASDHISAGA	0.448	0.689	Snapshot omitted
2	34	2	missense	ABCC12	p.T1251I	0.213		1.6	AEVIENGENF	LPEKLQAEVIENGEN	TIMKLPEKLQAEVIENGENFSVGER	0.605	0.556	Snapshot omitted
2	35	1	missense	SMAD4	p.G359E	0.268	0.460	572.9	YVDPSGEDRF	IVTVDGYVDPSGEDR	CPIVTVDGYVDPSGEDRFCLGQLSN	0.617	0.539	Snapshot omitted
2	36	2	missense	RELN	p.T1712M	0.182		4.2	MESSIYTSERF	MESSIYTSERFQNWK	VPPTIGCLHYMESSIYTSERFQNWK	0.430	0.631	Snapshot omitted
2	37	1	missense	VSIG10L	p.A188T	0.401	1.000	0.4	AETHSTASF	ETHSTASFPQQVGGP	ESKFSAETHSTASFPQQVGGPLAVL	0.634	0.397	Snapshot omitted
2	38	1	missense	LRCH4	p.A109V	0.186	0.320	13.3	NPVLGNLTAL	LNPVLGNLTALTYLN	YHNCLRCLNPVLGNLTALTYLNLSR	0.454	0.545	Snapshot omitted
2	39	1	missense	HNF4G	p.L195F	0.292	0.680	28.1	CESMKQQLF	QQLFVLVEWAKYIPA	DVCESMKQQLFVLVEWAKYIPAFCE	0.462	0.530	Snapshot omitted
2	40	1	inframe_del	SELPLG	p.Q135_T144del	0.432		10.0	ATEAQTTPL	TTQPAATEAQTTPLA	IQTTQPAATEAQTTPLAATEA	0.455	0.529	Snapshot omitted
2	41	2	missense	GBX2	p.K140M	0.239		1.9	QPLPGGGNFDM	GGNFDMAEALQADAE	APQPLPGGGNFDMAEALQADAEDGK	0.420	0.550	Snapshot omitted
2	42	1	missense	E4F1	p.R740Q	0.189		11.0	SEGTVLAAQ	EGTVLAAQAGTSGTE	ASAISEGTVLAAQAGTSGTEQATVT	0.241	0.647	Snapshot omitted
2	43	1	missense	GOLGA4	p.E744D	0.102	0.010	114.0	IIKEHDVSI	IIKEHDVSIQRTEKA	QQVDSIIKEHDVSIQRTEKALKDQI	0.447	0.521	Snapshot omitted
2	44	1	missense	PTOV1	p.G15D	0.225		19.0	APYRSGAGDPL	APYRSGAGDPLGGRG	VRPRRAPYRSGAGDPLGGRGRPPRP	0.506	0.462	Snapshot omitted
2	45	1	missense	WASHC2A	p.A12V	0.421		67.9	QELVPASEPVW	DQELVPASEPVWERP	MNRTTPDQELVPASEPVWERPWSVE	0.627	0.271	Snapshot omitted
2	46	1	missense	LSM8	p.N66D	0.161	0.090	269.0	RGDDVAVI	GLYIVRGDDVAVIGE	EQVVLGLYIVRGDDVAVIGEIDEET	0.451	0.507	Snapshot omitted
2	47	2	missense	LAMC3	p.R344H	0.316		1.6	RELFHSTGH	ECTFDRELFHSTGHG	SGRSEECTFDRELFHSTGHGGRCHH	0.365	0.569	Snapshot omitted
2	48	1	missense	PTPRS	p.V53M	0.279	0.060	13.3	VSGGVASFM	QIGVSGGVASFMCQA	PKDQIGVSGGVASFMCQATGDPKPR	0.412	0.535	Snapshot omitted
2	49	2	frameshift	CCDC6	p.E311Rfs*19	0.579	0.000	264.0	SVDSSPRV	EEKPSVDSSPRVSPA	LQRKLQRRWREEKPSVDSSPRVSPA	0.583	0.333	Snapshot omitted
2	50	2	missense	OLFML2B	p.V604G	0.341		5.0	GAYEEATPWRW	DGAYEEATPWRWQGH	YVAAWAMLHDGAYEEATPWRWQGHS	0.429	0.498	Snapshot omitted
2	51	1	missense	LMO7	p.T153R	0.266	0.540	530.7	LGQALRKAL	LNLKAFENLLGQALR	LNLKAFENLLGQALRKALEDSSFLK	0.328	0.554	Snapshot omitted
2	52	2	missense	PCOLCE2	p.V124G	0.606	1.000	0.5	KMMGQMISDA	GQMISDANTAGNGFM	ALVSSGNKMMGQMISDANTAGNGFM	0.460	0.425	Snapshot omitted
2	53	2	missense	BRCA2	p.W194R	0.332	0.480	315.7	DPDMSRSSSL	DPDMSRSSSLATPPT	LGAEVDPDMSRSSSLATPPTLSSTV	0.470	0.414	Snapshot omitted
2	54	2	missense	DMRT1	p.A37V	0.144	1.000	0.6	FGKASGVLV	FGKASGVLVGAASGS	GRAGGFGKASGVLVGAASGSSAGGS	0.310	0.540	Snapshot omitted
2	55	2	missense	MTMR7	p.R650W	0.241	0.420	4.0	SEDSGKDW	SGKDWDSDEAVFLTA	SGGEHAPSEDSGKDWDSDEAVFLTA	0.510	0.331	Snapshot omitted
2	56	1	missense	RFX1	p.Q238H	0.328	0.400	3.7	QVHGVHQSV	VHGVHQSVPVTQERS	VPQQLQVHGVHQSVPVTQERSVVQA	0.302	0.507	Snapshot omitted
2	57	1	missense	FHIP2B	p.D350N	0.277	0.280	32.9	FLGWFDYCNHL	NHLITEAHTVVADAL	AAFLGWFDYCNHLITEAHTVVADAL	0.388	0.445	Snapshot omitted
2	58	2	missense	NBPF15	p.R153K	0.277	0.220	67.5	AEGCKLTQHL	GCKLTQHLVQKLSPE	DLQEQLAEGCKLTQHLVQKLSPEND	0.405	0.427	Snapshot omitted
2	59	2	missense	GPD1	p.D103Y	0.308	0.500	4.1	HQFIGKICY	PHQFIGKICYQLKGH	LIFVVPHQFIGKICYQLKGHLKANA	0.522	0.270	Snapshot omitted
2	60	2	frameshift	APC	p.S1411Rfs*4	0.221		355.6	VQSEPCREW	ESRSIASSVQSEPCR	ESRSIASSVQSEPCREW	0.526	0.262	Snapshot omitted
2	61	2	missense	MYOCD	p.V491G	0.282	0.500	6.8	SPVHGCTEESL	SFGLHPSPVHGCTEE	DASPSFGLHPSPVHGCTEESLMSSL	0.413	0.389	Snapshot omitted
2	62	2	missense	VGF	p.H568N	0.366	1.000	1.8	NALPPSRHY	LRRRHYHNALPPSRH	PPSALRRRHYHNALPPSRHYPGREA	0.388	0.403	Snapshot omitted
2	63	2	missense	ZNF646	p.M1243I	0.276	0.290	7.9	KGFSNLISL	FSNLISLKNHRRIHA	QACSKGFSNLISLKNHRRIHADPRR	0.372	0.380	Snapshot omitted
2	64	2	missense	MTMR3	p.R541Q	0.315	0.530	25.4	SLLQAGNKA	QAGNKAFKNLLYSSQ	ERTCSVWSLLQAGNKAFKNLLYSSQ	0.369	0.382	Snapshot omitted
2	65	2	missense	ELF3	p.Q151H	0.319	0.370	481.0	HEALDPGPF	SWIIELLEKDGMAFH	SWIIELLEKDGMAFHEALDPGPFD	0.396	0.352	Snapshot omitted
2	66	2	missense	RHBDF1	p.E494K	0.144		31.6	SARKREKHSA	QDPQVHSFIRSARKR	RQDPQVHSFIRSARKREKHSACCVR	0.106	0.515	Snapshot omitted
2	67	2	missense	AASS	p.L88I	0.365	0.420	5.9	SEACIILGV	QEDISEACIILGVKR	AGGILQEDISEACIILGVKRPPEEK	0.371	0.353	Snapshot omitted
2	68	2	missense	GPSM3	p.Q117K	0.330		2.8	ILSHQCKRM	REQLYSTILSHQCKR	DREQLYSTILSHQCKRMEAQRSEPP	0.106	0.458	Snapshot omitted
2	69	2	missense	MDN1	p.R5381C	0.272	0.330	22.6	KPSKCQYQI	KDKIWLRRTKPSKCQ	RKDKIWLRRTKPSKCQYQICLAIDD	0.399	0.120	Snapshot omitted
2	70	2	missense	ZNF77	p.V455G	0.248	0.250	5.5	REHGRTHSG	HGRTHSGEKPYECNQ	FSCHSSLREHGRTHSGEKPYECNQ	0.280	0.221	Snapshot omitted
2	71	2	missense	BCLAF3	p.S6C	0.637	0.880	25.1	CRSPRWKHRSL	CRSPRWKHRSLSPVP	MARSRCRSPRWKHRSLSPVP	0.341	0.091	Snapshot omitted
2	72	2	missense	PODXL	p.Q350H	0.183		21.9	HTQSEKQLVL	AHESNWAKCEDLETH	AHESNWAKCEDLETHTQSEKQLVLN	0.310	0.091	Snapshot omitted
2	73	2	missense	ZNF77	p.V455M	0.239	0.250	5.5	REHMRTHSG	GKAFSCHSSLREHMR	CGKAFSCHSSLREHMRTHSGEKPYE	0.280	0.161	Snapshot omitted
2	74	2	missense	ZNF473	p.S434N	0.337	0.640	5.7	NECGKAFHRH	HQRVHSGEKPYKCNE	IHQRVHSGEKPYKCNECGKAFHRHT	0.216	0.107	Snapshot omitted
2	75	2	missense	ZNF473	p.S434R	0.333	0.640	5.7	RECGKAFHRH	HQRVHSGEKPYKCRE	IHQRVHSGEKPYKCRECGKAFHRHT	0.217	0.091	Snapshot omitted
2	76	2	missense	MED29	p.A123V	0.321	0.750	5.7	QLELCLRLV	LRLVHECLSQSCDSA	CDQLELCLRLVHECLSQSCDSAKHS	0.216	0.091	Snapshot omitted
2	77	2	missense	TRAF2	p.T113I	0.275	0.380	29.1	AVCPSDGCIW	IWKGTLKEYESCHEG	LPAVCPSDGCIWKGTLKEYESCHEG	0.049	0.134	Snapshot omitted
2	78	2	missense	THBS4	p.Y459C	0.312	0.600	0.8	VGWAGDGCI	GVGWAGDGCICGKDV	VTCVCGVGWAGDGCICGKDVDIDSY	0.010	0.107	Snapshot omitted
3	79	1	missense	ACOX1	p.H647D	0.048	0.110	37.2	AEVDESYKHL	NSPLNKAEVDESYKH	EWAKNSPLNKAEVDESYKHLKSLQS	0.739	0.523	Snapshot omitted
3	80	1	missense	EMP1	p.G148D	0.041	0.050	50.5	FIIDVLYLV	FSFIIDVLYLVLRKK	YILGWICFCFSFIIDVLYLVLRKK	0.853	0.274	Snapshot omitted
3	81	1	splice	PUM2	p.Q874R	0.111		81.7	IIDAFKGRV	AFKGRVFVLSTHPYG	LQFIIDAFKGRVFVLSTHPYGCRVI	0.502	0.722	Snapshot omitted
3	82	1	missense	H2AC7	p.I80T	0.013	0.100	496.7	TPRHLQLAI	ITPRHLQLAIRNDEE	AARDNKKTRITPRHLQLAIRNDEEL	0.694	0.532	Snapshot omitted
3	83	1	missense	SHANK1	p.P1668L	0.379		5.6	APQPGPDPL	AAPAPAAPQPGPDPL	AAPAPAAPQPGPDPLPGTDSGIEEV	0.618	0.527	Snapshot omitted
3	84	1	missense	MITF	p.M245T	0.019	0.240	94.0	LTDPALQM	TDPALQMANTLPVSG	SSYNEEILGLTDPALQMANTLPVSG	0.595	0.529	Snapshot omitted
3	85	2	missense	SLC6A15	p.L631F	0.263		0.7	FAIFPVPVV	FPVPVVFIVRRFNLI	VCVSLVVFAIFPVPVVFIVRRFNLI	0.622	0.475	Snapshot omitted
3	86	1	missense	IGLV3-1	p.S70N	0.014	0.100	14.1	YQDNKRPSGI	SPVLVIYQDNKRPSG	KPGQSPVLVIYQDNKRPSGIPERFS	0.439	0.631	Snapshot omitted
3	87	1	missense	AMER1	p.V663L	0.022	0.050	56.5	RPLGPSLMGL	LMGLAAGVSGTSQIS	EYQMRPLGPSLMGLAAGVSGTSQIS	0.626	0.444	Snapshot omitted
3	88	2	missense	CPXCR1	p.L100F	0.693		0.0	IPRKFVSHKPL	PRKFVSHKPLNDRSR	LLLQTPIPRKFVSHKPLNDRSRSHS	0.475	0.593	Snapshot omitted
3	89	1	missense	RPL3	p.M216K	0.024	0.060	1763.9	GQDEKIDVI	KIDVIGVTKGKGYKG	PVNQVFGQDEKIDVIGVTKGKGYKG	0.510	0.562	Snapshot omitted
3	90	1	missense	DNA2	p.H285R	0.038	0.050	54.5	IRRGYKTKY	IDVTVGVKIRRGYKT	LKGKIDVTVGVKIRRGYKTKYKIMP	0.231	0.714	Snapshot omitted
3	91	1	missense	MSANTD4	p.N270D	0.088		10.7	IVDSEKPSL	RLQIVDSEKPSLENE	EREKLRLQIVDSEKPSLENELGQGE	0.686	0.229	Snapshot omitted
3	92	2	missense	ASIC2	p.P17A	0.619		0.4	AALTGAGRF	AGRFRMAREEPAPAA	AGLPAAALTGAGRFRMAREEPAPAA	0.411	0.591	Snapshot omitted
3	93	1	missense	CPS1	p.T900I	0.224		1.4	SESMIEETL	EKTLKGLNSESMIEE	NMEKTLKGLNSESMIEETLKRAKEI	0.478	0.537	Snapshot omitted
3	94	1	missense	CCDC30	p.S358R	0.294		5.7	EELRHINQSL	PEEIVRLREELRHIN	TMKPEEIVRLREELRHINQSLLQS	0.477	0.536	Snapshot omitted
3	95	1	missense	TUBA4B	p.T69P	0.270	0.250	2.8	KLADQCPGL	PGLQGFLVFHSLGRG	DRIRKLADQCPGLQGFLVFHSLGRG	0.610	0.367	Snapshot omitted
3	96	1	missense	PLEKHG3	p.A147T	0.067	0.070	37.6	ALFGNIENIYT	NIENIYTLNSQLLRD	QVSALFGNIENIYTLNSQLLRDLDS	0.463	0.538	Snapshot omitted
3	97	1	missense	SOX9	p.S279N	0.020	0.080	4311.5	NSDVISNI	NSDVISNIETFDVNE	DFRDVDIGELNSDVISNIETFDVNE	0.493	0.505	Snapshot omitted
3	98	1	missense	METTL1	p.V8L	0.233		9.7	MAAETRNL	TRNLAGAEAPPPQKR	MAAETRNLAGAEAPPPQKRYYR	0.459	0.536	Snapshot omitted
3	99	1	missense	RPL3	p.M216V	0.022	0.060	1763.9	GQDEVIDVI	VIDVIGVTKGKGYKG	PVNQVFGQDEVIDVIGVTKGKGYKG	0.490	0.506	Snapshot omitted
3	100	1	missense	TBX3	p.P485L	0.019	0.370	18.9	GPLPGLGFAL	FALGLAGQQFFNGHP	QGPLPGLGFALGLAGQQFFNGHPLF	0.544	0.440	Snapshot omitted
3	101	1	missense	ZFHX4	p.A569T	0.201		1.4	TTAAHPSEI	TTAAHPSEIARGDED	FADASASKDSTTAAHPSEIARGDED	0.364	0.597	Snapshot omitted
3	102	1	missense	PTPRD	p.Q1601R	0.016	0.090	262.0	QTEDRYIFI	DRYIFIHDALLEAVT	QRNYMVQTEDRYIFIHDALLEAVT	0.426	0.540	Snapshot omitted
3	103	1	missense	KRT20	p.E377D	0.166		192.4	LEGDDVKTTEY	GDDVKTTEYQLSTLE	IATYRRLLEGDDVKTTEYQLSTLEE	0.462	0.508	Snapshot omitted
3	104	1	frameshift	SELENOP	p.L13Sfs*24	0.088		56.6	EEQRARTKAPY	EEQRARTKAPYVSNP	LCSSHREEQRARTKAPYVSNPQPGA	0.523	0.443	Snapshot omitted
3	105	1	missense	TRMT12	p.V23I	0.249		3.9	VAIVTEPRF	PVAVVAIVTEPRFTQ	RESGKPVAVVAIVTEPRFTQRYREY	0.445	0.515	Snapshot omitted
3	106	1	missense	TCF15	p.R80W	0.100	1.000	0.7	RQRQAANAW	VRQRQAANAWERDRT	GPVVVVRQRQAANAWERDRTQSVNT	0.439	0.515	Snapshot omitted
3	107	1	missense	METTL1	p.V8W	0.269		9.7	AETRNWAGA	AETRNWAGAEAPPPQ	MAAETRNWAGAEAPPPQKRYYR	0.303	0.603	Snapshot omitted
3	108	1	missense	PHF21B	p.A138T	0.108	0.670	1.6	QALAEPATL	TLASPLSSAGVAYAI	SQPQALAEPATLASPLSSAGVAYAI	0.568	0.361	Snapshot omitted
3	109	1	missense	METTL1	p.V8G	0.233		9.7	AETRNGAGA	AETRNGAGAEAPPPQ	MAAETRNGAGAEAPPPQKRYYR	0.401	0.540	Snapshot omitted
3	110	1	missense	DEF8	p.C390W	0.200	0.440	15.2	QAKGFVWEL	QAKGFVWELCREGDV	DCERCQAKGFVWELCREGDVLFPFD	0.519	0.418	Snapshot omitted
3	111	2	missense	CLEC2L	p.R68H	0.267		4.3	ALEDTTTHL	ALEDTTTHLLLGAIA	TSWKAALEDTTTHLLLGAIAVLLFA	0.609	0.265	Snapshot omitted
3	112	1	missense	DEF8	p.C390Y	0.196	0.440	15.2	QAKGFVYEL	QAKGFVYELCREGDV	DCERCQAKGFVYELCREGDVLFPFD	0.511	0.405	Snapshot omitted
3	113	1	missense	GOLGB1	p.E1411D	0.140		61.7	KEDDVSYL	EDDVSYLSGQLSEKE	ELQKLISKKEDDVSYLSGQLSEKEA	0.410	0.500	Snapshot omitted
3	114	1	missense	POLR2A	p.S1808N	0.013	0.050	66.1	SPSNPRYTP	SPSYSPSNPRYTPQS	SYSPTSPSYSPSNPRYTPQSPTYTP	0.434	0.456	Snapshot omitted
3	115	1	missense	MUC4	p.Q2605H	0.191	0.050	30.1				0.457	0.404	Snapshot omitted
3	116	1	missense	KIF1C	p.Q227L	0.265	0.320	251.1	AVFTIVFTL	LRCHDQLTGLDSEKV	SHAVFTIVFTLRCHDQLTGLDSEKV	0.552	0.222	Snapshot omitted
3	117	1	missense	CUX1	p.Q571R	0.011	0.060	294.5	KHNIGRRIF	GRRIFGHYVLGLSQG	KEQLIKHNIGRRIFGHYVLGLSQGS	0.321	0.489	Snapshot omitted
3	118	1	missense	MLXIPL	p.E648K	0.012	0.060	14.4	RPDSNKTK	TKNRRITHISAEQKR	SRGRPDSNKTKNRRITHISAEQKRR	0.231	0.535	Snapshot omitted
3	119	1	missense	AGAP4	p.T306A	0.091	0.070	39.7	VALCSNGVL	KKKYVALCSNGVLTY	WLKTWKKKYVALCSNGVLTYYSSLG	0.426	0.377	Snapshot omitted
3	120	1	missense	CAD	p.I1587M	0.244	0.500	39.5	ETWPSHLPM	LPMVAHAEQQTVAAV	HFETWPSHLPMVAHAEQQTVAAVLM	0.394	0.407	Snapshot omitted
3	121	1	missense	TMEM190	p.L56M	0.410	0.800	1.3	AIDSPNLCM	GGQAAIDSPNLCMRL	SCGGQAAIDSPNLCMRLRCCYRNGV	0.556	0.000	Snapshot omitted
3	122	1	missense	PCDHGB1	p.R635H	0.298	0.140	2.2	LGDRDAAHQRL	HQRLLVAVRDGGQPP	ARALGDRDAAHQRLLVAVRDGGQPP	0.409	0.365	Snapshot omitted
3	123	2	missense	SV2A	p.R419H	0.260		1.1	YQHWGVRAL	GTWYQHWGVRALSLG	IQSDTGTWYQHWGVRALSLGGQVWG	0.474	0.269	Snapshot omitted
3	124	2	missense	GUCY1A1	p.F288L	0.267		4.7	SLFCKTFPL	IPTSLFCKTFPLHFM	SLVIPTSLFCKTFPLHFMFDKDMTI	0.460	0.223	Snapshot omitted
3	125	2	missense	SPMIP6	p.V255L	0.293		2.2	SPALILPI	RNAVCCYNSPALILP	APARNAVCCYNSPALILPISEP	0.372	0.343	Snapshot omitted
3	126	2	missense	TET1	p.N100D	0.587		3.7	TCDGFTMAL	FQNPESLTCDGFTMA	TEVLFQNPESLTCDGFTMALRSTSL	0.483	0.120	Snapshot omitted
3	127	1	missense	TMEM207	p.P132L	0.184	1.000	0.2	VPAPCFGLL	QTPDLYPVPAPCFGL	QTPDLYPVPAPCFGLLGSPPPYEEI	0.475	0.105	Snapshot omitted
3	128	1	frameshift	APC	p.V450Gfs*4	0.290		355.6	VEHQICPAGVF	MPAPVEHQICPAGVF	NPMPAPVEHQICPAGVF	0.466	0.067	Snapshot omitted
3	129	1	splice	POMP	p.F55S	0.233	0.350	44.0	LSEKNSQL	PLELSEKNSQLNQDK	LLPSHPLELSEKNSQLNQDKMNFST	0.407	0.213	Snapshot omitted
3	130	1	missense	BICDL1	p.G161R	0.351	0.710	18.8	REWEGRVSEL	REREWEGRVSELESD	ELRRRFENREREWEGRVSELESDVK	0.433	0.107	Snapshot omitted
3	131	2	missense	SALL1	p.T745M	0.262		7.5	RAFTMKGNL	MKGNLKTHYSVHRAM	KCKICGRAFTMKGNLKTHYSVHRAM	0.361	0.250	Snapshot omitted
3	132	1	missense	H2AC16	p.S123N	0.280	0.500	342.5	LPKKTENHHKA	LPNIQAVLLPKKTEN	LPNIQAVLLPKKTENHHKAKGK	0.315	0.273	Snapshot omitted
3	133	1	missense	DENND4A	p.S167I	0.269	0.340	26.8	IPIKGESPPH	VTDICIIIPIKGESP	NTLAVTDICIIIPIKGESPPHTFCK	0.313	0.274	Snapshot omitted
3	134	2	missense	PITPNM3	p.E520D	0.366		6.4	MSDGSSHSE	RPGRRMSDGSSHSES	ASRFQRPGRRMSDGSSHSESSESSD	0.282	0.264	Snapshot omitted
3	135	2	missense	ZNF77	p.V455R	0.267		5.5	REHRRTHSG	GKAFSCHSSLREHRR	CGKAFSCHSSLREHRRTHSGEKPYE	0.217	0.244	Snapshot omitted
3	136	1	missense	POTEH	p.S41I	0.144	0.040	27.4	FAWCRGSGKI	SGKINVGTSGDHDDS	HCFAWCRGSGKINVGTSGDHDDSAM	0.120	0.265	Snapshot omitted
3	137	1	missense	ZNF880	p.Q406K	0.196	0.060	9.3	HRMHTGEKPY	HHRMHTGEKPYKCNE	FCLTNHHRMHTGEKPYKCNECGKAF	0.241	0.115	Snapshot omitted
3	138	1	missense	ZNF880	p.Q406R	0.203	0.070	9.3	RMHTGERPY	HHRMHTGERPYKCNE	FCLTNHHRMHTGERPYKCNECGKAF	0.241	0.099	Snapshot omitted
3	139	1	missense	MAGI3	p.K3N	0.269	0.330	11.8	NTLKKKKHW	SNTLKKKKHWLSKVQ	MSNTLKKKKHWLSKVQE	0.230	0.089	Snapshot omitted
3	140	1	missense	FIP1L1	p.R532Q	0.256	0.390	358.8	EERHRERQH	HRERQHREKEETRHK	REKEERHRERQHREKEETRHKSSRS	0.242	0.016	Snapshot omitted
4	141	1	frameshift	FRAT2	p.G116Lfs*94	0.015			SAPPAARAL	RRPQRAAGAVPARMA	RGGGRRRPQRAAGAVPARMAQGRGH⚠	0.729	1.000	Snapshot omitted
4	142	1	missense	MB21D2	p.D52V	0.015		4.1	YVDQRALEI	YVDQRALEIHTAKDF	EFTKHDQREYVDQRALEIHTAKDFI	0.990	0.585	Snapshot omitted
4	143	1	frameshift	TMEM271	p.G113Afs*120	0.057		0.6	RPGALHRLQL	ESGAAAGAPAAAPGA	EATPGESGAAAGAPAAAPGAGEQPE⚠	0.774	0.833	Snapshot omitted
4	144	1	frameshift	FOXK1	p.E371Afs*14	0.024		9.9	VPRSQAWEGVL	LSLNRYFIKVPRSQA	LSLNRYFIKVPRSQAWEGVLLANRP	0.722	0.865	Snapshot omitted
4	145	1	missense	MAB21L1	p.C215W	0.017		0.8	AEGFNLLSKEW	GFNLLSKEWHSLAGK	AEVKAEGFNLLSKEWHSLAGKQSSA	0.760	0.812	Snapshot omitted
4	146	1	missense	IGKC	p.Y79D	0.026	0.000	3597.8	KADDEKHKV	DEKHKVYACEVTHQG	SSTLTLSKADDEKHKVYACEVTHQG	0.872	0.651	Snapshot omitted
4	147	1	missense	PRUNE2	p.D1080V	0.023		11.6	YVDPSMMQL	GESSQSSYVDPSMMQ	EDDVGESSQSSYVDPSMMQLYNETN	0.934	0.481	Snapshot omitted
4	148	1	missense	MKI67	p.T2380P	0.029		144.7	RPPSAGKAM	KRPPSAGKAMDTPKP	EEEFLALRKRPPSAGKAMDTPKPAV	0.698	0.724	Snapshot omitted
4	149	1	frameshift	TM9SF2	p.L484Rfs*60	0.082		91.2	VPRRLLVHTL	LVLSWEGFCPLAASL	ALWFCISVPRRLLVHTLVLRRMPLN⚠	0.792	0.611	Snapshot omitted
4	150	1	missense	KIF3B	p.G285D	0.014		27.4	LVDDKSTHI	LGNVISALVDDKSTH	SLSALGNVISALVDDKSTHIPYRDS	0.843	0.520	Snapshot omitted
4	151	1	missense	CHD2	p.K344E	0.016	0.000	1877.5	KEEDEIKQW	EEDEIKQWLGKVSPE	GLKKLENFKKEEDEIKQWLGKVSPE	0.836	0.523	Snapshot omitted
4	152	1	missense	H2AC6	p.G107D	0.018	0.030	121.0	AQGDVLPNI	RVTIAQGDVLPNIQA	NKLLGRVTIAQGDVLPNIQAVLLPK	0.703	0.688	Snapshot omitted
4	153	1	missense	SOX9	p.A202D	0.033	0.000	4311.5	SPNDIFKAL	ISPNDIFKALQADSP	ATEQTHISPNDIFKALQADSPHSSS	0.892	0.387	Snapshot omitted
4	154	1	frameshift	FOXK1	p.P373Lfs*11	0.024		9.9	VPRSQEELL	LNRYFIKVPRSQEEL	LNRYFIKVPRSQEELLLGRGPFGE	0.802	0.536	Snapshot omitted
4	155	1	missense	FAT1	p.S4259T	0.022	0.000	7296.8	RPISYTPTI	VRPISYTPTIPSDSR	PPQVPVRPISYTPTIPSDSRNNLDR	0.624	0.725	Snapshot omitted
4	156	1	missense	PRKDC	p.D2937V	0.028	0.010	1846.0	KLYRSIGEYV	AKLYRSIGEYVVLRG	WVELAKLYRSIGEYVVLRGIFTSEI	0.896	0.321	Snapshot omitted
4	157	1	missense	SSR2	p.D39H	0.060		52.5	YAVEGRHLTL	HLTLQYNIYNVGSSA	LLNRYAVEGRHLTLQYNIYNVGSSA	0.785	0.520	Snapshot omitted
4	158	1	missense	CEACAM5	p.N610T	0.043	0.020	3850.8	YLSGATLNL	SSYLSGATLNLSCHS	ISPPDSSYLSGATLNLSCHSASNPS	0.771	0.538	Snapshot omitted
4	159	1	missense	ADGRL3	p.E563F	0.047		4.3	ALFESCEAV	IPALFESCEAVEARE	PSASSQIPALFESCEAVEAREIMWF	0.715	0.607	Snapshot omitted
4	160	1	missense	MYO15B	p.V2664T	0.027		171.8	LATASFLAL	DVSKLATASFLALMR	LSLSDDVSKLATASFLALMRFMGD	0.656	0.660	Snapshot omitted
4	161	1	missense	SCIMP	p.A105D	0.049	0.120	0.8	QEAPSQPPDTY	QPPDTYSLVNKVKNK	SPQEAPSQPPDTYSLVNKVKNKKTV	0.647	0.665	Snapshot omitted
4	162	1	missense	SOX9	p.I203V	0.016	0.000	4311.5	SPNAVFKAL	ISPNAVFKALQADSP	TEQTHISPNAVFKALQADSPHSSSG	0.768	0.500	Snapshot omitted
4	163	1	missense	SEM1	p.D16V	0.015	0.000	1141.0	EEVDEFEEF	LLEEVDEFEEFPAED	QPVDLGLLEEVDEFEEFPAEDWAGL	0.767	0.502	Snapshot omitted
4	164	1	missense	EXOC4	p.L69S	0.052	0.010	145.3	TESTTAIRTY	STTAIRTYQSITERI	DELIVQHYTESTTAIRTYQSITERI	0.686	0.607	Snapshot omitted
4	165	1	missense	STUM	p.A16V	0.010			AETAAAAAV	ETAAAAAVVAAADPR	SHKDAETAAAAAVVAAADPRGASSS	0.458	0.793	Snapshot omitted
4	166	1	missense	SF3B1	p.A861V	0.039	0.000	2396.6	IVDDLKDEV	KDEVEQYRKMVMETI	SRIVDDLKDEVEQYRKMVMETIEKI	0.728	0.546	Snapshot omitted
4	167	1	missense	ATP1A1	p.N754D	0.016	0.000	560.1	LLDDDFASI	DMILLDDDFASIVTG	SKQAADMILLDDDFASIVTGVEEGR	0.701	0.578	Snapshot omitted
4	168	1	missense	ELOB	p.G76W	0.011		74.1	APATVWLAF	ARPQAPATVWLAFRA	FTSQTARPQAPATVWLAFRADDTFE	0.644	0.639	Snapshot omitted
4	169	1	missense	NOS1	p.I87N	0.029		1.1	NASETHVVL	ALEVLRGNASETHVV	SYDSALEVLRGNASETHVVLILRGP	0.676	0.600	Snapshot omitted
4	170	1	missense	H1-5	p.K160T	0.019	0.000	352.2	TPKKATKPA	KTPKKATKPAAAGVK	KKAVKKTPKKATKPAAAGVKKVAKS	0.502	0.745	Snapshot omitted
4	171	1	missense	WRN	p.L161S	0.013	0.040	604.3	STDVANKKL	DFDIKLKNFVESTDV	LRDFDIKLKNFVESTDVANKKLKCT	0.636	0.633	Snapshot omitted
4	172	1	missense	KRT20	p.E377V	0.071		192.4	GVDVKTTEY	GVDVKTTEYQLSTLE	IATYRRLLEGVDVKTTEYQLSTLEE	0.702	0.555	Snapshot omitted
4	173	1	missense	HCN2	p.G805R	0.242		0.8	RPALPARRL	RTSPYGGLPAAPLAR	RTSPYGGLPAAPLARPALPARRLSR	0.730	0.514	Snapshot omitted
4	174	1	missense	CDH1	p.S635T	0.020		10186.8	AELTHGATANW	TSPFTAELTHGATAN	PNTSPFTAELTHGATANWTIQYNDP	0.724	0.521	Snapshot omitted
4	175	1	missense	H1-4	p.K159T	0.025		231.2	TPKKATKPA	KTPKKATKPAAAAGA	KKSAKKTPKKATKPAAAAGAKKAKS	0.492	0.743	Snapshot omitted
4	176	1	missense	ACTB	p.R116H	0.018	0.000	1681.4	HEKMTQIMF	HEKMTQIMFETFNTP	TEAPLNPKANHEKMTQIMFETFNTP	0.603	0.650	Snapshot omitted
4	177	1	missense	CGNL1	p.A109V	0.026		2.8	LQLPENPYV	VQPSPIRNLKQPLLH	EELQLPENPYVQPSPIRNLKQPLLH	0.598	0.654	Snapshot omitted
4	178	1	missense	DSP	p.H2363D	0.032		192.7	IEKGDGIRL	GDGIRLLEAQIATGG	AMNKELIEKGDGIRLLEAQIATGGI	0.591	0.659	Snapshot omitted
4	179	1	missense	DNAH10	p.I3606V	0.047		3.9	YVDPVIDNV	FRDVDEYVDPVIDNV	GTPFLFRDVDEYVDPVIDNVLEKNI	0.742	0.479	Snapshot omitted
4	180	1	missense	NKX2-2	p.S11L	0.013		1.0	FLVKDILDL	KTGFLVKDILDLPDT	MSLTNTKTGFLVKDILDLPDTNDEE	0.627	0.621	Snapshot omitted
4	181	1	missense	TSPAN15	p.V71D	0.032		96.1	LLGDVMFMV	GDVMFMVSFIGVLAS	APAIILILLGDVMFMVSFIGVLASL	0.834	0.277	Snapshot omitted
4	182	1	missense	DDR1	p.G707D	0.011	0.030	447.4	MENDDLNQF	NDDLNQFLSAHQLED	LCMITDYMENDDLNQFLSAHQLEDK	0.753	0.453	Snapshot omitted
4	183	1	missense	EEF2	p.E254D	0.017	0.000	2507.1	KVDDMMKKL	DDMMKKLWGDRYFDP	LGPAERAKKVDDMMKKLWGDRYFDP	0.739	0.472	Snapshot omitted
4	184	1	missense	NKX2-1	p.G89V	0.033		2.1	AAMQQHAVV	QHAVVHHGAVTAAYH	PPTAAMQQHAVVHHGAVTAAYHMTA	0.472	0.731	Snapshot omitted
4	185	1	missense	SOX9	p.N201D	0.018	0.000	4311.5	SPDAIFKAL	QTHISPDAIFKALQA	EEATEQTHISPDAIFKALQADSPHS	0.764	0.416	Snapshot omitted
4	186	1	missense	OPTN	p.F226V	0.039		78.0	SADGAKNYV	GAKNYVEHEELTVSQ	RSRSADGAKNYVEHEELTVSQLLL	0.727	0.471	Snapshot omitted
4	187	1	missense	IGHV3-72	p.A82V	0.028		11.6	YTTEYVASV	ANSYTTEYVASVKGR	RTRNKANSYTTEYVASVKGRFTISR	0.628	0.589	Snapshot omitted
4	188	1	missense	ACTB	p.E117D	0.048		1681.4	NPKANRDKM	RDKMTQIMFETFNTP	EAPLNPKANRDKMTQIMFETFNTPA	0.548	0.662	Snapshot omitted
4	189	1	missense	PRR36	p.P756H	0.082		4.3	TPHLENLPSL	PPPLQTASAPLTTPH	PPPLQTASAPLTTPHLENLPSLAPP	0.643	0.571	Snapshot omitted
4	190	1	missense	B2M	p.C100R	0.016	0.010	3207.6	YARRVNHVTL	RRVNHVTLSQPKIVK	FTPTEKDEYARRVNHVTLSQPKIVK	0.618	0.595	Snapshot omitted
4	191	1	splice	KIF13B	p.E19Q	0.026		88.7	RPMNRRQTDL	QTDLHTKCVVDVDAN	AVRIRPMNRRQTDLHTKCVVDVDAN	0.764	0.389	Snapshot omitted
4	192	1	missense	FAT1	p.E2921D	0.024		7296.8	TADIYKGTV	TADIYKGTVSEDDPQ	VNDSPPRFTADIYKGTVSEDDPQGG	0.586	0.620	Snapshot omitted
4	193	1	missense	TRAPPC14	p.L79R	0.034		7.5	AELATARAAL	LATARAALASVSAGG	RGAWAELATARAALASVSAGGGMPG	0.529	0.669	Snapshot omitted
4	194	1	missense	CFAP65	p.P650T	0.034		1.6	TIFPPTISV	TSDITIFPPTISVEP	FFFDGTSDITIFPPTISVEPVEVDF	0.677	0.518	Snapshot omitted
4	195	1	missense	KDM4B	p.N173I	0.015		27.0	TIIEGVITPYL	ITPYLYFGMWKTTFA	RECGTIIEGVITPYLYFGMWKTTFA	0.627	0.576	Snapshot omitted
4	196	1	missense	KDM4B	p.N173Y	0.016		27.0	TIIEGVYTPYL	YTPYLYFGMWKTTFA	RECGTIIEGVYTPYLYFGMWKTTFA	0.630	0.574	Snapshot omitted
4	197	1	missense	CFL1	p.V64I	0.016	0.010	585.0	TIDDPYATF	GKEILVGDVGQTIDD	EEGKEILVGDVGQTIDDPYATFVKM	0.703	0.480	Snapshot omitted
4	198	1	missense	TMEM176B	p.I160V	0.016		87.1	FIWQTEPFLYV	NSFIWQTEPFLYVDT	CVNSFIWQTEPFLYVDTVCDRSDPV	0.449	0.723	Snapshot omitted
4	199	1	missense	SOX9	p.H197Y	0.019	0.010	4311.5	AEAEEATEQTY	KNGQAEAEEATEQTY	KNGQAEAEEATEQTYISPNAIFKAL	0.756	0.387	Snapshot omitted
4	200	1	missense	NKX2-1	p.H90P	0.038		2.1	GPHGAVTAA	AVGPHGAVTAAYHMT	TAAMQQHAVGPHGAVTAAYHMTAAG	0.633	0.567	Snapshot omitted
4	201	1	missense	KCNT1	p.V984A	0.056		1.1	FAKDYMITI	LDTLLYQSFAKDYMI	SISMLDTLLYQSFAKDYMITITRLL	0.649	0.545	Snapshot omitted
4	202	1	missense	RPL3	p.I217N	0.011	0.000	1763.9	GQDEMNDVIGV	MNDVIGVTKGKGYKG	VNQVFGQDEMNDVIGVTKGKGYKGV	0.638	0.558	Snapshot omitted
4	203	1	missense	RIPOR3	p.R379W	0.015		8.2	WATSILSYL	QQPTQQALLLGGPWA	LQQPTQQALLLGGPWATSILSYLSD	0.627	0.569	Snapshot omitted
4	204	1	missense	C1orf127	p.A765D	0.017		1.7	SLWDPTGVLL	TQTLSLWDPTGVLLP	SSPPSTQTLSLWDPTGVLLPSLVEL	0.839	0.119	Snapshot omitted
4	205	1	missense	DICER1	p.S1896R	0.027	0.000	1016.7	GKFKGVGRRY	VGRRYRIAKSAAARR	GKGKFKGVGRRYRIAKSAAARRALR	0.328	0.781	Snapshot omitted
4	206	1	missense	CLIC1	p.N171D	0.042	0.030	197.5	FLDGDELTL	DEGVSQRKFLDGDEL	AEDEGVSQRKFLDGDELTLADCNLL	0.742	0.403	Snapshot omitted
4	207	1	missense	ELOB	p.T74P	0.014		74.1	APAPVGLAF	ARPQAPAPVGLAFRA	FTSQTARPQAPAPVGLAFRADDTFE	0.758	0.370	Snapshot omitted
4	208	1	missense	CARD11	p.D415V	0.011	0.000	134.1	EEKNVEMRI	RKQIRELEEKNVEMR	DKYRKQIRELEEKNVEMRIEMVRRE	0.542	0.643	Snapshot omitted
4	209	1	missense	TPT1	p.Y88D	0.048	0.000	2981.4	KEADKKYIKDY	NHHLQETSFTKEADK	MNHHLQETSFTKEADKKYIKDYMKS	0.658	0.523	Snapshot omitted
4	210	1	missense	LBR	p.S97P	0.017		194.8	RPASASHQA	PASASHQADIKEARR	PGRPPKSARRPASASHQADIKEARR	0.524	0.649	Snapshot omitted
4	211	1	missense	SOX9	p.Q195P	0.048	0.000	4311.5	EEATEPTHI	SVKNGQAEAEEATEP	SVKNGQAEAEEATEPTHISPNAIFK	0.543	0.633	Snapshot omitted
4	212	1	missense	CEACAM5	p.T317A	0.031	0.000	3850.8	AVYAEPPKPF	RTTVTTIAVYAEPPK	TGLNRTTVTTIAVYAEPPKPFITSN	0.435	0.710	Snapshot omitted
4	213	1	missense	KDM4B	p.N173F	0.017		27.0	TIIEGVFTPYL	FTPYLYFGMWKTTFA	RECGTIIEGVFTPYLYFGMWKTTFA	0.582	0.595	Snapshot omitted
4	214	1	missense	EWSR1	p.R506L	0.013		502.0	FPPRGPRGSL	PRGPRGSLGNPSGGG	DRGGFPPRGPRGSLGNPSGGGNVQH	0.705	0.440	Snapshot omitted
4	215	1	missense	MITF	p.L244S	0.013		94.0	SMDPALQM	SMDPALQMANTLPVS	ESSYNEEILGSMDPALQMANTLPVS	0.637	0.532	Snapshot omitted
4	216	1	missense	MAP7	p.M269T	0.031	0.030	133.3	STDRPKLFV	PIIMPYKAAHSRNST	PIIMPYKAAHSRNSTDRPKLFVTPP	0.506	0.658	Snapshot omitted
4	217	1	missense	CACNA1G	p.P1906L	0.028		1.8	SPDSPKLGAL	PDSPKLGALHPAAHA	EGPDSPDSPKLGALHPAAHARSASH	0.677	0.478	Snapshot omitted
4	218	1	missense	SF3B1	p.E870A	0.012	0.000	2396.6	MVMATIEKI	YRKMVMATIEKIMGN	DEAEQYRKMVMATIEKIMGNLGAAD	0.579	0.589	Snapshot omitted
4	219	1	missense	ACTB	p.N115D	0.013	0.000	1681.4	NPKADREKM	DREKMTQIMFETFNT	LTEAPLNPKADREKMTQIMFETFNT	0.540	0.623	Snapshot omitted
4	220	1	missense	ADGRL3	p.C566W	0.042		4.3	WEAVEAREIMW	LEESWEAVEAREIMW	SSQIPALEESWEAVEAREIMWFKTR	0.550	0.609	Snapshot omitted
4	221	1	missense	APCDD1	p.K162L	0.023		44.7	AVAELLGQQV	TEAVAELLGQQVNRT	QVICHTEAVAELLGQQVNRTCPGFL	0.655	0.492	Snapshot omitted
4	222	1	missense	ATRX	p.N365D	0.011		2947.2	TADMNSSYV	KKLIETTADMNSSYV	MIKKAKKLIETTADMNSSYVKFLK	0.689	0.443	Snapshot omitted
4	223	1	missense	COPG2	p.C647R	0.044		10.5	AETEYFVRRI	VRRIKHMFTNHIVFQ	TEAETEYFVRRIKHMFTNHIVFQFD	0.616	0.538	Snapshot omitted
4	224	1	missense	KCNT1	p.V984M	0.063		1.1	FMKDYMITI	MLDTLLYQSFMKDYM	SISMLDTLLYQSFMKDYMITITRLL	0.636	0.513	Snapshot omitted
4	225	1	missense	SOX9	p.E134D	0.017		4311.5	YPHLHNADL	YPHLHNADLSKTLGK	KLADQYPHLHNADLSKTLGKLWRLL	0.698	0.420	Snapshot omitted
4	226	1	missense	NCOA5	p.P501S	0.014		91.3	GPRSGAPSQGL	NMGPRSGAPSQGLFG	GGSAQNMGPRSGAPSQGLFGQPSSR	0.688	0.435	Snapshot omitted
4	227	1	missense	BNC2	p.A650V	0.042		13.4	IIDTVDEF	EKEIIDTVDEFDDED	MPVKIEKEIIDTVDEFDDEDDDPND	0.633	0.511	Snapshot omitted
4	228	1	splice	COX4I1	p.E80D	0.050		451.5	KVDLYRIKF	KVDLYRIKFKESFAE	WSSLSMDEKVDLYRIKFKESFAEMN	0.637	0.505	Snapshot omitted
4	229	1	missense	FMN2	p.S1536P	0.081		7.8	SLLPYIVSYYL	LLPYIVSYYLRNFDE	KSSDNSRSLLPYIVSYYLRNFDEDA	0.568	0.579	Snapshot omitted
4	230	1	missense	MTDH	p.E500D	0.047		342.2	TSDPADVLV	DVLVKNSQPIKTLPP	LKTISTSDPADVLVKNSQPIKTLPP	0.609	0.535	Snapshot omitted
4	231	1	missense	CEACAM5	p.K492T	0.034	0.000	3850.8	TTITVSAEL	RTTVTTITVSAELPK	SASGHSRTTVTTITVSAELPKPSIS	0.583	0.564	Snapshot omitted
4	232	1	missense	ELOB	p.V75L	0.012		74.1	APATLGLAF	ARPQAPATLGLAFRA	FTSQTARPQAPATLGLAFRADDTFE	0.664	0.464	Snapshot omitted
4	233	1	frameshift	FYB1	p.W648Sfs*14	0.125		7.6	QEKSNTSVL	VQEKSNTSVLGDFED	GSTLQVQEKSNTSVLGDFEDVKGKR	0.663	0.463	Snapshot omitted
4	234	1	missense	CEACAM5	p.S404N	0.013	0.000	3850.8	SVDHNDPVI	NDPVILNVLYGPDDP	GIQNELSVDHNDPVILNVLYGPDDP	0.596	0.545	Snapshot omitted
4	235	1	missense	SOX9	p.P433L	0.010	0.010	4311.5	LPHYSPSYL	SPSYLPITRSQYDYT	PFNLPHYSPSYLPITRSQYDYTDH	0.659	0.461	Snapshot omitted
4	236	1	missense	LINGO1	p.V571D	0.022		2.1	FLGDVLFCL	IATTMGFISFLGDVL	LIIATTMGFISFLGDVLFCLVLLFL	0.719	0.361	Snapshot omitted
4	237	1	missense	MUC17	p.N4116T	0.045		103.5	TAVPTTTTI	SFPTVTTTAVPTTTT	TTSFPTVTTTAVPTTTTIKSNPTST	0.709	0.374	Snapshot omitted
4	238	1	missense	CACNA1G	p.K1905R	0.028		1.8	SPDSPRPGAL	PDSPRPGALHPAAHA	VEGPDSPDSPRPGALHPAAHARSAS	0.640	0.483	Snapshot omitted
4	239	1	missense	OSBPL7	p.L306M	0.022		22.6	MAQKVHSSL	PPTDYAHLQRSFWAM	PPTDYAHLQRSFWAMAQKVHSSLSS	0.669	0.442	Snapshot omitted
4	240	1	missense	TGM6	p.Y216D	0.013	0.060	3.1	NPIDVTRVI	DVTRVISAMVNSNND	TDVSCRHNPIDVTRVISAMVNSNND	0.599	0.531	Snapshot omitted
4	241	1	missense	ACTG1	p.N115D	0.032	0.000	920.2	NPKADREKM	DREKMTQIMFETFNT	LTEAPLNPKADREKMTQIMFETFNT	0.511	0.614	Snapshot omitted
4	242	1	missense	SOX9	p.Y84H	0.015	0.000	4311.5	VLKGHDWTL	SQVLKGHDWTLVPMP	IREAVSQVLKGHDWTLVPMPVRVNG	0.519	0.607	Snapshot omitted
4	243	1	missense	MUC13	p.S118P	0.025	0.010	130.1	SLATPDIITA	TPDIITASSPNDGLI	STTNVNSLATPDIITASSPNDGLIT	0.578	0.549	Snapshot omitted
4	244	1	missense	FAT1	p.G2653D	0.059	0.010	7296.8	LSDVITTKESL	LEINKLSDVITTKES	VKENLEINKLSDVITTKESLIGLEN	0.591	0.533	Snapshot omitted
4	245	1	missense	ATXN2	p.A1078T	0.016	0.010	134.1	HPSHVQPTY	VFTIHPSHVQPTYTN	QQTVFTIHPSHVQPTYTNPPHMAHV	0.391	0.693	Snapshot omitted
4	246	1	splice	PIK3CB	p.Q207H	0.019		1001.7	FENCHDVFSF	GGKLIVAVHFENCHD	YGGKLIVAVHFENCHDVFSFQVSPN	0.443	0.658	Snapshot omitted
4	247	1	splice	B4GALT5	p.L265R	0.034		15.3	KLDKYMYRL	DKYMYRLPYTEFFGG	HFATKLDKYMYRLPYTEFFGGVSGL	0.697	0.378	Snapshot omitted
4	248	1	missense	DNM2	p.F258Y	0.015		216.3	LAAERKYFL	GKKDIRAALAAERKY	GKKDIRAALAAERKYFLSHPAYRHM	0.548	0.571	Snapshot omitted
4	249	1	missense	ZMYND8	p.G533D	0.018	0.010	124.6	TTDSILNL	TDSILNLNLDRSKAE	TTKTDKTSTTDSILNLNLDRSKAEM	0.663	0.423	Snapshot omitted
4	250	1	missense	OSBPL7	p.L306R	0.022		22.6	RAQKVHSSL	PPTDYAHLQRSFWAR	PPTDYAHLQRSFWARAQKVHSSLSS	0.585	0.523	Snapshot omitted

Tier	#	Samples	Type	Gene	HGVSp	DNA AF	RNA AF	RNA_TPM	Best CD8 epitope	Best CD4 epitope	HLA ON Tier \| Rank	CD8 Probability	CD4 Probability	IGV
1	1	2	missense	STAG3	p.D893Y	0.237	0.140	4.4	YVFKHYNKF	AASYVFKHYNKFYND	Tier 1 \| #1	0.715	0.716	Snapshot omitted
1	2	2	missense	KLHL26	p.V126M	0.255	0.030	6.5	FAYSAEMTL	RGLRHIIDFAYSAEM	Tier 1 \| #2	0.756	0.477	Snapshot omitted
1	3	2	stop_lost	PRKDC	p.4129Gext14	0.320	0.470	1846.0	SADRKHYIV	WEPWMGGLWESADRK	Tier 1 \| #3	0.650	0.590	Snapshot omitted
1	4	2	missense	FOXL2	p.A234E	0.635	0.570	4.8	AEGPGSPGAAA	AAAAAAAAAAAAEGP	Tier 1 \| #4	0.454	0.706	Snapshot omitted
1	5	2	missense	PKD1	p.R3130W	0.465	0.600	49.9	YEILVKTGWGW	TGWGWGSGTTAHVGI	Tier 1 \| #5	0.507	0.654	Snapshot omitted
1	6	2	missense	CARF	p.D455N	0.248	0.250	5.1	KPNEVPERHNL	RKFVERELFKPNEVP	Tier 1 \| #6	0.598	0.533	Snapshot omitted
1	7	2	missense	FNDC1	p.R336H	0.272	0.200	6.7	TVYEFAVHI	PDTVYEFAVHISQGE	Tier 1 \| #7	0.561	0.545	Snapshot omitted
1	8	2	missense	MYH7B	p.A549D	0.198	0.460	3.5	ASDDSFRAKL	DDSFRAKLYDNHAGK	Tier 1 \| #8	0.578	0.514	Snapshot omitted
1	9	2	missense	GALK2	p.G450D	0.280	0.600	45.4	KPGDGALVL	PEKQSLFATKPGDGA	Tier 1 \| #9	0.760	0.120	Snapshot omitted
1	10	2	frameshift	BCAS1	p.V461Gfs*28	0.206		50.2	KEKKANQEPL	KSKEVESALQTGTST	Tier 1 \| #10	0.580	0.495	Snapshot omitted
1	11	2	missense	TRIM14	p.V68L	0.283	0.170	29.3	LALEAAVHL	HPVGLALEAAVHLQK	Tier 1 \| #11	0.668	0.354	Snapshot omitted
1	12	2	missense	DAGLB	p.V28G	0.454	0.150	15.2	FELVGRVLW	SDDLVFPGFFELVGR	Tier 1 \| #12	0.536	0.481	Snapshot omitted
1	13	2	missense	GPR157	p.P314A	0.348	0.600	5.7	SPAGTPKAA	AGTPKAAAPSKPGES	Tier 1 \| #13	0.448	0.543	Snapshot omitted
1	14	2	missense	SLC39A10	p.M740I	0.239	0.570	17.2	SAIMAYIGM	VKQAIVYNLLSAIMA	Tier 1 \| #14	0.443	0.533	Snapshot omitted
1	15	2	missense	HMCN1	p.V4127I	0.248	0.170	3.1	ILSSGSLQI	RAIVESIRQRILSSG	Tier 1 \| #15	0.444	0.526	Snapshot omitted
1	16	2	missense	WWC1	p.D1064E	0.267	0.590	32.1	GELQTEKMM	KGELQTEKMMRAAAK	Tier 1 \| #16	0.441	0.525	Snapshot omitted
1	17	2	missense	SMARCA4	p.D1177Y	0.291	0.370	3386.1	SADTVIIFDSY	ADTVIIFDSYWNPHQ	Tier 1 \| #17	0.516	0.444	Snapshot omitted
1	18	2	missense	TLN2	p.E2148Q	0.343	0.640	63.3	ALQATIECI	DEATRGTRALQATIE	Tier 1 \| #18	0.395	0.524	Snapshot omitted
1	19	2	missense	PAN2	p.E602A	0.276		30.6	AASGKGNL	LGLILADSDAASGKG	Tier 1 \| #19	0.310	0.565	Snapshot omitted
1	20	2	missense	SSTR4	p.L351I	0.218	1.000	1.4	PLDYYATAI	EPLDYYATAIKSKGG	Tier 1 \| #20	0.383	0.499	Snapshot omitted
1	21	2	missense	HGF	p.C149F	0.427		17.0	SITKSGIKF	KSGIKFQPWSSMIPH	Tier 1 \| #21	0.312	0.536	Snapshot omitted
1	22	2	missense	WDFY1	p.V390I	0.236	0.270	40.1	GTDRIIKI	TDRIIKIWDMTPVVG	Tier 1 \| #22	0.426	0.437	Snapshot omitted
1	23	2	missense	KMT2E	p.T897P	0.167	0.250	110.3	YAPPTHTDI	YAPPTHTDITPMDPS	Tier 1 \| #23	0.599	0.119	Snapshot omitted
1	24	2	missense	ZNF704	p.S127G	0.324	0.240	29.1	VPSSTSGSGY	GSWKEGGCVPSSTSG	Tier 1 \| #24	0.288	0.523	Snapshot omitted
1	25	2	splice	CASP10	p.N240K	0.242	0.380	6.7	HAGSKGNRA	QNKHAGSKGNRATNG	Tier 1 \| #25	0.106	0.534	Snapshot omitted
2	26	2	frameshift	PLXNA4	p.I1045Lfs*45	0.216		2.8	YVEDPTIVRL	EDPTIVRLSQNGALS	Tier 2 \| #26	0.731	0.826	Snapshot omitted
2	27	2	missense	TTN	p.D20524Y	0.280		1.9	YLIQDLPRV	VYLIQDLPRVELQIK	Tier 2 \| #27	0.915	0.483	Snapshot omitted
2	28	1	missense	PRRC2C	p.Y392N	0.202	0.430	141.0	VPNGKGPSF	PAQPSVAKVPNGKGP	Tier 2 \| #28	0.787	0.491	Snapshot omitted
2	29	1	missense	PLEKHG2	p.R1293W	0.176		12.0	YISQSLARW	RWQGPGGGAPAASRG	Tier 2 \| #29	0.303	0.810	Snapshot omitted
2	30	1	missense	ADPRHL1	p.R1805W	0.365	0.920	4.5	QERAWEQGW	WEQALTSGMAPRAWE	Tier 2 \| #30	0.672	0.508	Snapshot omitted
2	31	2	missense	DNASE1L2	p.L11F	0.336		1.3	GPRALLAAF	PRALLAAFWALEAAG	Tier 2 \| #31	0.654	0.526	Snapshot omitted
2	32	2	missense	SLFN12L	p.C535G	0.576		3.0	GVMTKIFSL	KVGVMTKIFSLSPEG	Tier 2 \| #32	0.622	0.546	Snapshot omitted
2	33	2	missense	GAPDHS	p.L174I	0.339		1.2	VVESTGVYI	YISIQAASDHISAGA	Tier 2 \| #33	0.448	0.689	Snapshot omitted
2	34	2	missense	ABCC12	p.T1251I	0.213		1.6	AEVIENGENF	LPEKLQAEVIENGEN	Tier 2 \| #34	0.605	0.556	Snapshot omitted
2	35	1	missense	SMAD4	p.G359E	0.268	0.460	572.9	YVDPSGEDRF	IVTVDGYVDPSGEDR	Tier 2 \| #35	0.617	0.539	Snapshot omitted
2	36	2	missense	RELN	p.T1712M	0.182		4.2	MESSIYTSERF	MESSIYTSERFQNWK	Tier 2 \| #36	0.430	0.631	Snapshot omitted
2	37	1	missense	VSIG10L	p.A188T	0.401	1.000	0.4	AETHSTASF	ETHSTASFPQQVGGP	Tier 2 \| #37	0.634	0.397	Snapshot omitted
2	38	1	missense	LRCH4	p.A109V	0.186	0.320	13.3	NPVLGNLTAL	LNPVLGNLTALTYLN	Tier 2 \| #38	0.454	0.545	Snapshot omitted
2	39	1	missense	HNF4G	p.L195F	0.292	0.680	28.1	CESMKQQLF	QQLFVLVEWAKYIPA	Tier 2 \| #39	0.462	0.530	Snapshot omitted
2	40	1	inframe_del	SELPLG	p.Q135_T144del	0.432		10.0	ATEAQTTPL	TTQPAATEAQTTPLA	Tier 2 \| #40	0.455	0.529	Snapshot omitted
2	41	2	missense	GBX2	p.K140M	0.239		1.9	QPLPGGGNFDM	GGNFDMAEALQADAE	Tier 2 \| #41	0.420	0.550	Snapshot omitted
2	42	1	missense	E4F1	p.R740Q	0.189		11.0	SEGTVLAAQ	EGTVLAAQAGTSGTE	Tier 2 \| #42	0.241	0.647	Snapshot omitted
2	43	1	missense	GOLGA4	p.E744D	0.102	0.010	114.0	IIKEHDVSI	IIKEHDVSIQRTEKA	Tier 2 \| #43	0.447	0.521	Snapshot omitted
2	44	1	missense	PTOV1	p.G15D	0.225		19.0	APYRSGAGDPL	APYRSGAGDPLGGRG	Tier 2 \| #44	0.506	0.462	Snapshot omitted
2	45	1	missense	WASHC2A	p.A12V	0.421		67.9	QELVPASEPVW	DQELVPASEPVWERP	Tier 2 \| #45	0.627	0.271	Snapshot omitted
2	46	1	missense	LSM8	p.N66D	0.161	0.090	269.0	RGDDVAVI	GLYIVRGDDVAVIGE	Tier 2 \| #46	0.451	0.507	Snapshot omitted
2	47	2	missense	LAMC3	p.R344H	0.316		1.6	RELFHSTGH	ECTFDRELFHSTGHG	Tier 2 \| #47	0.365	0.569	Snapshot omitted
2	48	1	missense	PTPRS	p.V53M	0.279	0.060	13.3	VSGGVASFM	QIGVSGGVASFMCQA	Tier 2 \| #48	0.412	0.535	Snapshot omitted
2	49	2	frameshift	CCDC6	p.E311Rfs*19	0.579	0.000	264.0	SVDSSPRV	EEKPSVDSSPRVSPA	Tier 2 \| #49	0.583	0.333	Snapshot omitted
2	50	2	missense	OLFML2B	p.V604G	0.341		5.0	GAYEEATPWRW	DGAYEEATPWRWQGH	Tier 2 \| #50	0.429	0.498	Snapshot omitted
2	51	1	missense	LMO7	p.T153R	0.266	0.540	530.7	LGQALRKAL	LNLKAFENLLGQALR	Tier 2 \| #51	0.328	0.554	Snapshot omitted
2	52	2	missense	PCOLCE2	p.V124G	0.606	1.000	0.5	KMMGQMISDA	GQMISDANTAGNGFM	Tier 2 \| #52	0.460	0.425	Snapshot omitted
2	53	2	missense	BRCA2	p.W194R	0.332	0.480	315.7	DPDMSRSSSL	DPDMSRSSSLATPPT	Tier 2 \| #53	0.470	0.414	Snapshot omitted
2	54	2	missense	DMRT1	p.A37V	0.144	1.000	0.6	FGKASGVLV	FGKASGVLVGAASGS	Tier 2 \| #54	0.310	0.540	Snapshot omitted
2	55	2	missense	MTMR7	p.R650W	0.241	0.420	4.0	SEDSGKDW	SGKDWDSDEAVFLTA	Tier 2 \| #55	0.510	0.331	Snapshot omitted
2	56	1	missense	RFX1	p.Q238H	0.328	0.400	3.7	QVHGVHQSV	VHGVHQSVPVTQERS	Tier 2 \| #56	0.302	0.507	Snapshot omitted
2	57	1	missense	FHIP2B	p.D350N	0.277	0.280	32.9	FLGWFDYCNHL	NHLITEAHTVVADAL	Tier 2 \| #57	0.388	0.445	Snapshot omitted
2	58	2	missense	NBPF15	p.R153K	0.277	0.220	67.5	AEGCKLTQHL	GCKLTQHLVQKLSPE	Tier 2 \| #58	0.405	0.427	Snapshot omitted
2	59	2	missense	GPD1	p.D103Y	0.308	0.500	4.1	HQFIGKICY	PHQFIGKICYQLKGH	Tier 2 \| #59	0.522	0.270	Snapshot omitted
2	60	2	frameshift	APC	p.S1411Rfs*4	0.221		355.6	VQSEPCREW	ESRSIASSVQSEPCR	Tier 2 \| #60	0.526	0.262	Snapshot omitted
2	61	2	missense	MYOCD	p.V491G	0.282	0.500	6.8	SPVHGCTEESL	SFGLHPSPVHGCTEE	Tier 2 \| #61	0.413	0.389	Snapshot omitted
2	62	2	missense	VGF	p.H568N	0.366	1.000	1.8	NALPPSRHY	LRRRHYHNALPPSRH	Tier 2 \| #62	0.388	0.403	Snapshot omitted
2	63	2	missense	ZNF646	p.M1243I	0.276	0.290	7.9	KGFSNLISL	FSNLISLKNHRRIHA	Tier 2 \| #63	0.372	0.380	Snapshot omitted
2	64	2	missense	MTMR3	p.R541Q	0.315	0.530	25.4	SLLQAGNKA	QAGNKAFKNLLYSSQ	Tier 2 \| #64	0.369	0.382	Snapshot omitted
2	65	2	missense	ELF3	p.Q151H	0.319	0.370	481.0	HEALDPGPF	SWIIELLEKDGMAFH	Tier 2 \| #65	0.396	0.352	Snapshot omitted
2	66	2	missense	RHBDF1	p.E494K	0.144		31.6	SARKREKHSA	QDPQVHSFIRSARKR	Tier 2 \| #66	0.106	0.515	Snapshot omitted
2	67	2	missense	AASS	p.L88I	0.365	0.420	5.9	SEACIILGV	QEDISEACIILGVKR	Tier 2 \| #67	0.371	0.353	Snapshot omitted
2	68	2	missense	GPSM3	p.Q117K	0.330		2.8	ILSHQCKRM	REQLYSTILSHQCKR	Tier 2 \| #68	0.106	0.458	Snapshot omitted
2	69	2	missense	MDN1	p.R5381C	0.272	0.330	22.6	KPSKCQYQI	KDKIWLRRTKPSKCQ	Tier 2 \| #69	0.399	0.120	Snapshot omitted
2	70	2	missense	ZNF77	p.V455G	0.248	0.250	5.5	REHGRTHSG	HGRTHSGEKPYECNQ	Tier 2 \| #70	0.280	0.221	Snapshot omitted
2	71	2	missense	BCLAF3	p.S6C	0.637	0.880	25.1	CRSPRWKHRSL	CRSPRWKHRSLSPVP	Tier 2 \| #71	0.341	0.091	Snapshot omitted
2	72	2	missense	PODXL	p.Q350H	0.183		21.9	HTQSEKQLVL	AHESNWAKCEDLETH	Tier 2 \| #72	0.310	0.091	Snapshot omitted
2	73	2	missense	ZNF77	p.V455M	0.239	0.250	5.5	REHMRTHSG	GKAFSCHSSLREHMR	Tier 2 \| #73	0.280	0.161	Snapshot omitted
2	74	2	missense	ZNF473	p.S434N	0.337	0.640	5.7	NECGKAFHRH	HQRVHSGEKPYKCNE	Tier 2 \| #74	0.216	0.107	Snapshot omitted
2	75	2	missense	ZNF473	p.S434R	0.333	0.640	5.7	RECGKAFHRH	HQRVHSGEKPYKCRE	Tier 2 \| #75	0.217	0.091	Snapshot omitted
2	76	2	missense	MED29	p.A123V	0.321	0.750	5.7	QLELCLRLV	LRLVHECLSQSCDSA	Tier 2 \| #76	0.216	0.091	Snapshot omitted
2	77	2	missense	TRAF2	p.T113I	0.275	0.380	29.1	AVCPSDGCIW	IWKGTLKEYESCHEG	Tier 2 \| #77	0.049	0.134	Snapshot omitted
2	78	2	missense	THBS4	p.Y459C	0.312	0.600	0.8	VGWAGDGCI	GVGWAGDGCICGKDV	Tier 2 \| #78	0.010	0.107	Snapshot omitted
3	79	1	missense	ACOX1	p.H647D	0.048	0.110	37.2	AEVDESYKHL	NSPLNKAEVDESYKH	Tier 3 \| #79	0.739	0.523	Snapshot omitted
3	80	1	missense	EMP1	p.G148D	0.041	0.050	50.5	FIIDVLYLV	FSFIIDVLYLVLRKK	Tier 3 \| #80	0.853	0.274	Snapshot omitted
3	81	1	splice	PUM2	p.Q874R	0.111		81.7	IIDAFKGRV	AFKGRVFVLSTHPYG	Tier 3 \| #81	0.502	0.722	Snapshot omitted
3	82	1	missense	H2AC7	p.I80T	0.013	0.100	496.7	TPRHLQLAI	ITPRHLQLAIRNDEE	Tier 3 \| #82	0.694	0.532	Snapshot omitted
3	83	1	missense	SHANK1	p.P1668L	0.379		5.6	APQPGPDPL	AAPAPAAPQPGPDPL	Tier 3 \| #83	0.618	0.527	Snapshot omitted
3	84	1	missense	MITF	p.M245T	0.019	0.240	94.0	LTDPALQM	TDPALQMANTLPVSG	Tier 3 \| #84	0.595	0.529	Snapshot omitted
3	85	2	missense	SLC6A15	p.L631F	0.263		0.7	FAIFPVPVV	FPVPVVFIVRRFNLI	Tier 3 \| #85	0.622	0.475	Snapshot omitted
3	86	1	missense	IGLV3-1	p.S70N	0.014	0.100	14.1	YQDNKRPSGI	SPVLVIYQDNKRPSG	Tier 3 \| #86	0.439	0.631	Snapshot omitted
3	87	1	missense	AMER1	p.V663L	0.022	0.050	56.5	RPLGPSLMGL	LMGLAAGVSGTSQIS	Tier 3 \| #87	0.626	0.444	Snapshot omitted
3	88	2	missense	CPXCR1	p.L100F	0.693		0.0	IPRKFVSHKPL	PRKFVSHKPLNDRSR	Tier 3 \| #88	0.475	0.593	Snapshot omitted
3	89	1	missense	RPL3	p.M216K	0.024	0.060	1763.9	GQDEKIDVI	KIDVIGVTKGKGYKG	Tier 3 \| #89	0.510	0.562	Snapshot omitted
3	90	1	missense	DNA2	p.H285R	0.038	0.050	54.5	IRRGYKTKY	IDVTVGVKIRRGYKT	Tier 3 \| #90	0.231	0.714	Snapshot omitted
3	91	1	missense	MSANTD4	p.N270D	0.088		10.7	IVDSEKPSL	RLQIVDSEKPSLENE	Tier 3 \| #91	0.686	0.229	Snapshot omitted
3	92	2	missense	ASIC2	p.P17A	0.619		0.4	AALTGAGRF	AGRFRMAREEPAPAA	Tier 3 \| #92	0.411	0.591	Snapshot omitted
3	93	1	missense	CPS1	p.T900I	0.224		1.4	SESMIEETL	EKTLKGLNSESMIEE	Tier 3 \| #93	0.478	0.537	Snapshot omitted
3	94	1	missense	CCDC30	p.S358R	0.294		5.7	EELRHINQSL	PEEIVRLREELRHIN	Tier 3 \| #94	0.477	0.536	Snapshot omitted
3	95	1	missense	TUBA4B	p.T69P	0.270	0.250	2.8	KLADQCPGL	PGLQGFLVFHSLGRG	Tier 3 \| #95	0.610	0.367	Snapshot omitted
3	96	1	missense	PLEKHG3	p.A147T	0.067	0.070	37.6	ALFGNIENIYT	NIENIYTLNSQLLRD	Tier 3 \| #96	0.463	0.538	Snapshot omitted
3	97	1	missense	SOX9	p.S279N	0.020	0.080	4311.5	NSDVISNI	NSDVISNIETFDVNE	Tier 3 \| #97	0.493	0.505	Snapshot omitted
3	98	1	missense	METTL1	p.V8L	0.233		9.7	MAAETRNL	TRNLAGAEAPPPQKR	Tier 3 \| #98	0.459	0.536	Snapshot omitted
3	99	1	missense	RPL3	p.M216V	0.022	0.060	1763.9	GQDEVIDVI	VIDVIGVTKGKGYKG	Tier 3 \| #99	0.490	0.506	Snapshot omitted
3	100	1	missense	TBX3	p.P485L	0.019	0.370	18.9	GPLPGLGFAL	FALGLAGQQFFNGHP	Tier 3 \| #100	0.544	0.440	Snapshot omitted
3	101	1	missense	ZFHX4	p.A569T	0.201		1.4	TTAAHPSEI	TTAAHPSEIARGDED	Tier 3 \| #101	0.364	0.597	Snapshot omitted
3	102	1	missense	PTPRD	p.Q1601R	0.016	0.090	262.0	QTEDRYIFI	DRYIFIHDALLEAVT	Tier 3 \| #102	0.426	0.540	Snapshot omitted
3	103	1	missense	KRT20	p.E377D	0.166		192.4	LEGDDVKTTEY	GDDVKTTEYQLSTLE	Tier 3 \| #103	0.462	0.508	Snapshot omitted
3	104	1	frameshift	SELENOP	p.L13Sfs*24	0.088		56.6	EEQRARTKAPY	EEQRARTKAPYVSNP	Tier 3 \| #104	0.523	0.443	Snapshot omitted
3	105	1	missense	TRMT12	p.V23I	0.249		3.9	VAIVTEPRF	PVAVVAIVTEPRFTQ	Tier 3 \| #105	0.445	0.515	Snapshot omitted
3	106	1	missense	TCF15	p.R80W	0.100	1.000	0.7	RQRQAANAW	VRQRQAANAWERDRT	Tier 3 \| #106	0.439	0.515	Snapshot omitted
3	107	1	missense	METTL1	p.V8W	0.269		9.7	AETRNWAGA	AETRNWAGAEAPPPQ	Tier 3 \| #107	0.303	0.603	Snapshot omitted
3	108	1	missense	PHF21B	p.A138T	0.108	0.670	1.6	QALAEPATL	TLASPLSSAGVAYAI	Tier 3 \| #108	0.568	0.361	Snapshot omitted
3	109	1	missense	METTL1	p.V8G	0.233		9.7	AETRNGAGA	AETRNGAGAEAPPPQ	Tier 3 \| #109	0.401	0.540	Snapshot omitted
3	110	1	missense	DEF8	p.C390W	0.200	0.440	15.2	QAKGFVWEL	QAKGFVWELCREGDV	Tier 3 \| #110	0.519	0.418	Snapshot omitted
3	111	2	missense	CLEC2L	p.R68H	0.267		4.3	ALEDTTTHL	ALEDTTTHLLLGAIA	Tier 3 \| #111	0.609	0.265	Snapshot omitted
3	112	1	missense	DEF8	p.C390Y	0.196	0.440	15.2	QAKGFVYEL	QAKGFVYELCREGDV	Tier 3 \| #112	0.511	0.405	Snapshot omitted
3	113	1	missense	GOLGB1	p.E1411D	0.140		61.7	KEDDVSYL	EDDVSYLSGQLSEKE	Tier 3 \| #113	0.410	0.500	Snapshot omitted
3	114	1	missense	POLR2A	p.S1808N	0.013	0.050	66.1	SPSNPRYTP	SPSYSPSNPRYTPQS	Tier 3 \| #114	0.434	0.456	Snapshot omitted
3	115	1	missense	MUC4	p.Q2605H	0.191	0.050	30.1			Tier 3 \| #115	0.457	0.404	Snapshot omitted
3	116	1	missense	KIF1C	p.Q227L	0.265	0.320	251.1	AVFTIVFTL	LRCHDQLTGLDSEKV	Tier 3 \| #116	0.552	0.222	Snapshot omitted
3	117	1	missense	CUX1	p.Q571R	0.011	0.060	294.5	KHNIGRRIF	GRRIFGHYVLGLSQG	Tier 3 \| #117	0.321	0.489	Snapshot omitted
3	118	1	missense	MLXIPL	p.E648K	0.012	0.060	14.4	RPDSNKTK	TKNRRITHISAEQKR	Tier 3 \| #118	0.231	0.535	Snapshot omitted
3	119	1	missense	AGAP4	p.T306A	0.091	0.070	39.7	VALCSNGVL	KKKYVALCSNGVLTY	Tier 3 \| #119	0.426	0.377	Snapshot omitted
3	120	1	missense	CAD	p.I1587M	0.244	0.500	39.5	ETWPSHLPM	LPMVAHAEQQTVAAV	Tier 3 \| #120	0.394	0.407	Snapshot omitted
3	121	1	missense	TMEM190	p.L56M	0.410	0.800	1.3	AIDSPNLCM	GGQAAIDSPNLCMRL	Tier 3 \| #121	0.556	0.000	Snapshot omitted
3	122	1	missense	PCDHGB1	p.R635H	0.298	0.140	2.2	LGDRDAAHQRL	HQRLLVAVRDGGQPP	Tier 3 \| #122	0.409	0.365	Snapshot omitted
3	123	2	missense	SV2A	p.R419H	0.260		1.1	YQHWGVRAL	GTWYQHWGVRALSLG	Tier 3 \| #123	0.474	0.269	Snapshot omitted
3	124	2	missense	GUCY1A1	p.F288L	0.267		4.7	SLFCKTFPL	IPTSLFCKTFPLHFM	Tier 3 \| #124	0.460	0.223	Snapshot omitted
3	125	2	missense	SPMIP6	p.V255L	0.293		2.2	SPALILPI	RNAVCCYNSPALILP	Tier 3 \| #125	0.372	0.343	Snapshot omitted
3	126	2	missense	TET1	p.N100D	0.587		3.7	TCDGFTMAL	FQNPESLTCDGFTMA	Tier 3 \| #126	0.483	0.120	Snapshot omitted
3	127	1	missense	TMEM207	p.P132L	0.184	1.000	0.2	VPAPCFGLL	QTPDLYPVPAPCFGL	Tier 3 \| #127	0.475	0.105	Snapshot omitted
3	128	1	frameshift	APC	p.V450Gfs*4	0.290		355.6	VEHQICPAGVF	MPAPVEHQICPAGVF	Tier 3 \| #128	0.466	0.067	Snapshot omitted
3	129	1	splice	POMP	p.F55S	0.233	0.350	44.0	LSEKNSQL	PLELSEKNSQLNQDK	Tier 3 \| #129	0.407	0.213	Snapshot omitted
3	130	1	missense	BICDL1	p.G161R	0.351	0.710	18.8	REWEGRVSEL	REREWEGRVSELESD	Tier 3 \| #130	0.433	0.107	Snapshot omitted
3	131	2	missense	SALL1	p.T745M	0.262		7.5	RAFTMKGNL	MKGNLKTHYSVHRAM	Tier 3 \| #131	0.361	0.250	Snapshot omitted
3	132	1	missense	H2AC16	p.S123N	0.280	0.500	342.5	LPKKTENHHKA	LPNIQAVLLPKKTEN	Tier 3 \| #132	0.315	0.273	Snapshot omitted
3	133	1	missense	DENND4A	p.S167I	0.269	0.340	26.8	IPIKGESPPH	VTDICIIIPIKGESP	Tier 3 \| #133	0.313	0.274	Snapshot omitted
3	134	2	missense	PITPNM3	p.E520D	0.366		6.4	MSDGSSHSE	RPGRRMSDGSSHSES	Tier 3 \| #134	0.282	0.264	Snapshot omitted
3	135	2	missense	ZNF77	p.V455R	0.267		5.5	REHRRTHSG	GKAFSCHSSLREHRR	Tier 3 \| #135	0.217	0.244	Snapshot omitted
3	136	1	missense	POTEH	p.S41I	0.144	0.040	27.4	FAWCRGSGKI	SGKINVGTSGDHDDS	Tier 3 \| #136	0.120	0.265	Snapshot omitted
3	137	1	missense	ZNF880	p.Q406K	0.196	0.060	9.3	HRMHTGEKPY	HHRMHTGEKPYKCNE	Tier 3 \| #137	0.241	0.115	Snapshot omitted
3	138	1	missense	ZNF880	p.Q406R	0.203	0.070	9.3	RMHTGERPY	HHRMHTGERPYKCNE	Tier 3 \| #138	0.241	0.099	Snapshot omitted
3	139	1	missense	MAGI3	p.K3N	0.269	0.330	11.8	NTLKKKKHW	SNTLKKKKHWLSKVQ	Tier 3 \| #139	0.230	0.089	Snapshot omitted
3	140	1	missense	FIP1L1	p.R532Q	0.256	0.390	358.8	EERHRERQH	HRERQHREKEETRHK	Tier 3 \| #140	0.242	0.016	Snapshot omitted
4	141	1	frameshift	FRAT2	p.G116Lfs*94	0.015			SAPPAARAL	RRPQRAAGAVPARMA	Tier 4 \| #141	0.729	1.000	Snapshot omitted
4	142	1	missense	MB21D2	p.D52V	0.015		4.1	YVDQRALEI	YVDQRALEIHTAKDF	Tier 4 \| #142	0.990	0.585	Snapshot omitted
4	143	1	frameshift	TMEM271	p.G113Afs*120	0.057		0.6	RPGALHRLQL	ESGAAAGAPAAAPGA	Tier 4 \| #143	0.774	0.833	Snapshot omitted
4	144	1	frameshift	FOXK1	p.E371Afs*14	0.024		9.9	VPRSQAWEGVL	LSLNRYFIKVPRSQA	Tier 4 \| #144	0.722	0.865	Snapshot omitted
4	145	1	missense	MAB21L1	p.C215W	0.017		0.8	AEGFNLLSKEW	GFNLLSKEWHSLAGK	Tier 4 \| #145	0.760	0.812	Snapshot omitted
4	146	1	missense	IGKC	p.Y79D	0.026	0.000	3597.8	KADDEKHKV	DEKHKVYACEVTHQG	Tier 4 \| #146	0.872	0.651	Snapshot omitted
4	147	1	missense	PRUNE2	p.D1080V	0.023		11.6	YVDPSMMQL	GESSQSSYVDPSMMQ	Tier 4 \| #147	0.934	0.481	Snapshot omitted
4	148	1	missense	MKI67	p.T2380P	0.029		144.7	RPPSAGKAM	KRPPSAGKAMDTPKP	Tier 4 \| #148	0.698	0.724	Snapshot omitted
4	149	1	frameshift	TM9SF2	p.L484Rfs*60	0.082		91.2	VPRRLLVHTL	LVLSWEGFCPLAASL	Tier 4 \| #149	0.792	0.611	Snapshot omitted
4	150	1	missense	KIF3B	p.G285D	0.014		27.4	LVDDKSTHI	LGNVISALVDDKSTH	Tier 4 \| #150	0.843	0.520	Snapshot omitted
4	151	1	missense	CHD2	p.K344E	0.016	0.000	1877.5	KEEDEIKQW	EEDEIKQWLGKVSPE	Tier 4 \| #151	0.836	0.523	Snapshot omitted
4	152	1	missense	H2AC6	p.G107D	0.018	0.030	121.0	AQGDVLPNI	RVTIAQGDVLPNIQA	Tier 4 \| #152	0.703	0.688	Snapshot omitted
4	153	1	missense	SOX9	p.A202D	0.033	0.000	4311.5	SPNDIFKAL	ISPNDIFKALQADSP	Tier 4 \| #153	0.892	0.387	Snapshot omitted
4	154	1	frameshift	FOXK1	p.P373Lfs*11	0.024		9.9	VPRSQEELL	LNRYFIKVPRSQEEL	Tier 4 \| #154	0.802	0.536	Snapshot omitted
4	155	1	missense	FAT1	p.S4259T	0.022	0.000	7296.8	RPISYTPTI	VRPISYTPTIPSDSR	Tier 4 \| #155	0.624	0.725	Snapshot omitted
4	156	1	missense	PRKDC	p.D2937V	0.028	0.010	1846.0	KLYRSIGEYV	AKLYRSIGEYVVLRG	Tier 4 \| #156	0.896	0.321	Snapshot omitted
4	157	1	missense	SSR2	p.D39H	0.060		52.5	YAVEGRHLTL	HLTLQYNIYNVGSSA	Tier 4 \| #157	0.785	0.520	Snapshot omitted
4	158	1	missense	CEACAM5	p.N610T	0.043	0.020	3850.8	YLSGATLNL	SSYLSGATLNLSCHS	Tier 4 \| #158	0.771	0.538	Snapshot omitted
4	159	1	missense	ADGRL3	p.E563F	0.047		4.3	ALFESCEAV	IPALFESCEAVEARE	Tier 4 \| #159	0.715	0.607	Snapshot omitted
4	160	1	missense	MYO15B	p.V2664T	0.027		171.8	LATASFLAL	DVSKLATASFLALMR	Tier 4 \| #160	0.656	0.660	Snapshot omitted
4	161	1	missense	SCIMP	p.A105D	0.049	0.120	0.8	QEAPSQPPDTY	QPPDTYSLVNKVKNK	Tier 4 \| #161	0.647	0.665	Snapshot omitted
4	162	1	missense	SOX9	p.I203V	0.016	0.000	4311.5	SPNAVFKAL	ISPNAVFKALQADSP	Tier 4 \| #162	0.768	0.500	Snapshot omitted
4	163	1	missense	SEM1	p.D16V	0.015	0.000	1141.0	EEVDEFEEF	LLEEVDEFEEFPAED	Tier 4 \| #163	0.767	0.502	Snapshot omitted
4	164	1	missense	EXOC4	p.L69S	0.052	0.010	145.3	TESTTAIRTY	STTAIRTYQSITERI	Tier 4 \| #164	0.686	0.607	Snapshot omitted
4	165	1	missense	STUM	p.A16V	0.010			AETAAAAAV	ETAAAAAVVAAADPR	Tier 4 \| #165	0.458	0.793	Snapshot omitted
4	166	1	missense	SF3B1	p.A861V	0.039	0.000	2396.6	IVDDLKDEV	KDEVEQYRKMVMETI	Tier 4 \| #166	0.728	0.546	Snapshot omitted
4	167	1	missense	ATP1A1	p.N754D	0.016	0.000	560.1	LLDDDFASI	DMILLDDDFASIVTG	Tier 4 \| #167	0.701	0.578	Snapshot omitted
4	168	1	missense	ELOB	p.G76W	0.011		74.1	APATVWLAF	ARPQAPATVWLAFRA	Tier 4 \| #168	0.644	0.639	Snapshot omitted
4	169	1	missense	NOS1	p.I87N	0.029		1.1	NASETHVVL	ALEVLRGNASETHVV	Tier 4 \| #169	0.676	0.600	Snapshot omitted
4	170	1	missense	H1-5	p.K160T	0.019	0.000	352.2	TPKKATKPA	KTPKKATKPAAAGVK	Tier 4 \| #170	0.502	0.745	Snapshot omitted
4	171	1	missense	WRN	p.L161S	0.013	0.040	604.3	STDVANKKL	DFDIKLKNFVESTDV	Tier 4 \| #171	0.636	0.633	Snapshot omitted
4	172	1	missense	KRT20	p.E377V	0.071		192.4	GVDVKTTEY	GVDVKTTEYQLSTLE	Tier 4 \| #172	0.702	0.555	Snapshot omitted
4	173	1	missense	HCN2	p.G805R	0.242		0.8	RPALPARRL	RTSPYGGLPAAPLAR	Tier 4 \| #173	0.730	0.514	Snapshot omitted
4	174	1	missense	CDH1	p.S635T	0.020		10186.8	AELTHGATANW	TSPFTAELTHGATAN	Tier 4 \| #174	0.724	0.521	Snapshot omitted
4	175	1	missense	H1-4	p.K159T	0.025		231.2	TPKKATKPA	KTPKKATKPAAAAGA	Tier 4 \| #175	0.492	0.743	Snapshot omitted
4	176	1	missense	ACTB	p.R116H	0.018	0.000	1681.4	HEKMTQIMF	HEKMTQIMFETFNTP	Tier 4 \| #176	0.603	0.650	Snapshot omitted
4	177	1	missense	CGNL1	p.A109V	0.026		2.8	LQLPENPYV	VQPSPIRNLKQPLLH	Tier 4 \| #177	0.598	0.654	Snapshot omitted
4	178	1	missense	DSP	p.H2363D	0.032		192.7	IEKGDGIRL	GDGIRLLEAQIATGG	Tier 4 \| #178	0.591	0.659	Snapshot omitted
4	179	1	missense	DNAH10	p.I3606V	0.047		3.9	YVDPVIDNV	FRDVDEYVDPVIDNV	Tier 4 \| #179	0.742	0.479	Snapshot omitted
4	180	1	missense	NKX2-2	p.S11L	0.013		1.0	FLVKDILDL	KTGFLVKDILDLPDT	Tier 4 \| #180	0.627	0.621	Snapshot omitted
4	181	1	missense	TSPAN15	p.V71D	0.032		96.1	LLGDVMFMV	GDVMFMVSFIGVLAS	Tier 4 \| #181	0.834	0.277	Snapshot omitted
4	182	1	missense	DDR1	p.G707D	0.011	0.030	447.4	MENDDLNQF	NDDLNQFLSAHQLED	Tier 4 \| #182	0.753	0.453	Snapshot omitted
4	183	1	missense	EEF2	p.E254D	0.017	0.000	2507.1	KVDDMMKKL	DDMMKKLWGDRYFDP	Tier 4 \| #183	0.739	0.472	Snapshot omitted
4	184	1	missense	NKX2-1	p.G89V	0.033		2.1	AAMQQHAVV	QHAVVHHGAVTAAYH	Tier 4 \| #184	0.472	0.731	Snapshot omitted
4	185	1	missense	SOX9	p.N201D	0.018	0.000	4311.5	SPDAIFKAL	QTHISPDAIFKALQA	Tier 4 \| #185	0.764	0.416	Snapshot omitted
4	186	1	missense	OPTN	p.F226V	0.039		78.0	SADGAKNYV	GAKNYVEHEELTVSQ	Tier 4 \| #186	0.727	0.471	Snapshot omitted
4	187	1	missense	IGHV3-72	p.A82V	0.028		11.6	YTTEYVASV	ANSYTTEYVASVKGR	Tier 4 \| #187	0.628	0.589	Snapshot omitted
4	188	1	missense	ACTB	p.E117D	0.048		1681.4	NPKANRDKM	RDKMTQIMFETFNTP	Tier 4 \| #188	0.548	0.662	Snapshot omitted
4	189	1	missense	PRR36	p.P756H	0.082		4.3	TPHLENLPSL	PPPLQTASAPLTTPH	Tier 4 \| #189	0.643	0.571	Snapshot omitted
4	190	1	missense	B2M	p.C100R	0.016	0.010	3207.6	YARRVNHVTL	RRVNHVTLSQPKIVK	Tier 4 \| #190	0.618	0.595	Snapshot omitted
4	191	1	splice	KIF13B	p.E19Q	0.026		88.7	RPMNRRQTDL	QTDLHTKCVVDVDAN	Tier 4 \| #191	0.764	0.389	Snapshot omitted
4	192	1	missense	FAT1	p.E2921D	0.024		7296.8	TADIYKGTV	TADIYKGTVSEDDPQ	Tier 4 \| #192	0.586	0.620	Snapshot omitted
4	193	1	missense	TRAPPC14	p.L79R	0.034		7.5	AELATARAAL	LATARAALASVSAGG	Tier 4 \| #193	0.529	0.669	Snapshot omitted
4	194	1	missense	CFAP65	p.P650T	0.034		1.6	TIFPPTISV	TSDITIFPPTISVEP	Tier 4 \| #194	0.677	0.518	Snapshot omitted
4	195	1	missense	KDM4B	p.N173I	0.015		27.0	TIIEGVITPYL	ITPYLYFGMWKTTFA	Tier 4 \| #195	0.627	0.576	Snapshot omitted
4	196	1	missense	KDM4B	p.N173Y	0.016		27.0	TIIEGVYTPYL	YTPYLYFGMWKTTFA	Tier 4 \| #196	0.630	0.574	Snapshot omitted
4	197	1	missense	CFL1	p.V64I	0.016	0.010	585.0	TIDDPYATF	GKEILVGDVGQTIDD	Tier 4 \| #197	0.703	0.480	Snapshot omitted
4	198	1	missense	TMEM176B	p.I160V	0.016		87.1	FIWQTEPFLYV	NSFIWQTEPFLYVDT	Tier 4 \| #198	0.449	0.723	Snapshot omitted
4	199	1	missense	SOX9	p.H197Y	0.019	0.010	4311.5	AEAEEATEQTY	KNGQAEAEEATEQTY	Tier 4 \| #199	0.756	0.387	Snapshot omitted
4	200	1	missense	NKX2-1	p.H90P	0.038		2.1	GPHGAVTAA	AVGPHGAVTAAYHMT	Tier 4 \| #200	0.633	0.567	Snapshot omitted
4	201	1	missense	KCNT1	p.V984A	0.056		1.1	FAKDYMITI	LDTLLYQSFAKDYMI	Tier 4 \| #201	0.649	0.545	Snapshot omitted
4	202	1	missense	RPL3	p.I217N	0.011	0.000	1763.9	GQDEMNDVIGV	MNDVIGVTKGKGYKG	Tier 4 \| #202	0.638	0.558	Snapshot omitted
4	203	1	missense	RIPOR3	p.R379W	0.015		8.2	WATSILSYL	QQPTQQALLLGGPWA	Tier 4 \| #203	0.627	0.569	Snapshot omitted
4	204	1	missense	C1orf127	p.A765D	0.017		1.7	SLWDPTGVLL	TQTLSLWDPTGVLLP	Tier 4 \| #204	0.839	0.119	Snapshot omitted
4	205	1	missense	DICER1	p.S1896R	0.027	0.000	1016.7	GKFKGVGRRY	VGRRYRIAKSAAARR	Tier 4 \| #205	0.328	0.781	Snapshot omitted
4	206	1	missense	CLIC1	p.N171D	0.042	0.030	197.5	FLDGDELTL	DEGVSQRKFLDGDEL	Tier 4 \| #206	0.742	0.403	Snapshot omitted
4	207	1	missense	ELOB	p.T74P	0.014		74.1	APAPVGLAF	ARPQAPAPVGLAFRA	Tier 4 \| #207	0.758	0.370	Snapshot omitted
4	208	1	missense	CARD11	p.D415V	0.011	0.000	134.1	EEKNVEMRI	RKQIRELEEKNVEMR	Tier 4 \| #208	0.542	0.643	Snapshot omitted
4	209	1	missense	TPT1	p.Y88D	0.048	0.000	2981.4	KEADKKYIKDY	NHHLQETSFTKEADK	Tier 4 \| #209	0.658	0.523	Snapshot omitted
4	210	1	missense	LBR	p.S97P	0.017		194.8	RPASASHQA	PASASHQADIKEARR	Tier 4 \| #210	0.524	0.649	Snapshot omitted
4	211	1	missense	SOX9	p.Q195P	0.048	0.000	4311.5	EEATEPTHI	SVKNGQAEAEEATEP	Tier 4 \| #211	0.543	0.633	Snapshot omitted
4	212	1	missense	CEACAM5	p.T317A	0.031	0.000	3850.8	AVYAEPPKPF	RTTVTTIAVYAEPPK	Tier 4 \| #212	0.435	0.710	Snapshot omitted
4	213	1	missense	KDM4B	p.N173F	0.017		27.0	TIIEGVFTPYL	FTPYLYFGMWKTTFA	Tier 4 \| #213	0.582	0.595	Snapshot omitted
4	214	1	missense	EWSR1	p.R506L	0.013		502.0	FPPRGPRGSL	PRGPRGSLGNPSGGG	Tier 4 \| #214	0.705	0.440	Snapshot omitted
4	215	1	missense	MITF	p.L244S	0.013		94.0	SMDPALQM	SMDPALQMANTLPVS	Tier 4 \| #215	0.637	0.532	Snapshot omitted
4	216	1	missense	MAP7	p.M269T	0.031	0.030	133.3	STDRPKLFV	PIIMPYKAAHSRNST	Tier 4 \| #216	0.506	0.658	Snapshot omitted
4	217	1	missense	CACNA1G	p.P1906L	0.028		1.8	SPDSPKLGAL	PDSPKLGALHPAAHA	Tier 4 \| #217	0.677	0.478	Snapshot omitted
4	218	1	missense	SF3B1	p.E870A	0.012	0.000	2396.6	MVMATIEKI	YRKMVMATIEKIMGN	Tier 4 \| #218	0.579	0.589	Snapshot omitted
4	219	1	missense	ACTB	p.N115D	0.013	0.000	1681.4	NPKADREKM	DREKMTQIMFETFNT	Tier 4 \| #219	0.540	0.623	Snapshot omitted
4	220	1	missense	ADGRL3	p.C566W	0.042		4.3	WEAVEAREIMW	LEESWEAVEAREIMW	Tier 4 \| #220	0.550	0.609	Snapshot omitted
4	221	1	missense	APCDD1	p.K162L	0.023		44.7	AVAELLGQQV	TEAVAELLGQQVNRT	Tier 4 \| #221	0.655	0.492	Snapshot omitted
4	222	1	missense	ATRX	p.N365D	0.011		2947.2	TADMNSSYV	KKLIETTADMNSSYV	Tier 4 \| #222	0.689	0.443	Snapshot omitted
4	223	1	missense	COPG2	p.C647R	0.044		10.5	AETEYFVRRI	VRRIKHMFTNHIVFQ	Tier 4 \| #223	0.616	0.538	Snapshot omitted
4	224	1	missense	KCNT1	p.V984M	0.063		1.1	FMKDYMITI	MLDTLLYQSFMKDYM	Tier 4 \| #224	0.636	0.513	Snapshot omitted
4	225	1	missense	SOX9	p.E134D	0.017		4311.5	YPHLHNADL	YPHLHNADLSKTLGK	Tier 4 \| #225	0.698	0.420	Snapshot omitted
4	226	1	missense	NCOA5	p.P501S	0.014		91.3	GPRSGAPSQGL	NMGPRSGAPSQGLFG	Tier 4 \| #226	0.688	0.435	Snapshot omitted
4	227	1	missense	BNC2	p.A650V	0.042		13.4	IIDTVDEF	EKEIIDTVDEFDDED	Tier 4 \| #227	0.633	0.511	Snapshot omitted
4	228	1	splice	COX4I1	p.E80D	0.050		451.5	KVDLYRIKF	KVDLYRIKFKESFAE	Tier 4 \| #228	0.637	0.505	Snapshot omitted
4	229	1	missense	FMN2	p.S1536P	0.081		7.8	SLLPYIVSYYL	LLPYIVSYYLRNFDE	Tier 4 \| #229	0.568	0.579	Snapshot omitted
4	230	1	missense	MTDH	p.E500D	0.047		342.2	TSDPADVLV	DVLVKNSQPIKTLPP	Tier 4 \| #230	0.609	0.535	Snapshot omitted
4	231	1	missense	CEACAM5	p.K492T	0.034	0.000	3850.8	TTITVSAEL	RTTVTTITVSAELPK	Tier 4 \| #231	0.583	0.564	Snapshot omitted
4	232	1	missense	ELOB	p.V75L	0.012		74.1	APATLGLAF	ARPQAPATLGLAFRA	Tier 4 \| #232	0.664	0.464	Snapshot omitted
4	233	1	frameshift	FYB1	p.W648Sfs*14	0.125		7.6	QEKSNTSVL	VQEKSNTSVLGDFED	Tier 4 \| #233	0.663	0.463	Snapshot omitted
4	234	1	missense	CEACAM5	p.S404N	0.013	0.000	3850.8	SVDHNDPVI	NDPVILNVLYGPDDP	Tier 4 \| #234	0.596	0.545	Snapshot omitted
4	235	1	missense	SOX9	p.P433L	0.010	0.010	4311.5	LPHYSPSYL	SPSYLPITRSQYDYT	Tier 4 \| #235	0.659	0.461	Snapshot omitted
4	236	1	missense	LINGO1	p.V571D	0.022		2.1	FLGDVLFCL	IATTMGFISFLGDVL	Tier 4 \| #236	0.719	0.361	Snapshot omitted
4	237	1	missense	MUC17	p.N4116T	0.045		103.5	TAVPTTTTI	SFPTVTTTAVPTTTT	Tier 4 \| #237	0.709	0.374	Snapshot omitted
4	238	1	missense	CACNA1G	p.K1905R	0.028		1.8	SPDSPRPGAL	PDSPRPGALHPAAHA	Tier 4 \| #238	0.640	0.483	Snapshot omitted
4	239	1	missense	OSBPL7	p.L306M	0.022		22.6	MAQKVHSSL	PPTDYAHLQRSFWAM	Tier 4 \| #239	0.669	0.442	Snapshot omitted
4	240	1	missense	TGM6	p.Y216D	0.013	0.060	3.1	NPIDVTRVI	DVTRVISAMVNSNND	Tier 4 \| #240	0.599	0.531	Snapshot omitted
4	241	1	missense	ACTG1	p.N115D	0.032	0.000	920.2	NPKADREKM	DREKMTQIMFETFNT	Tier 4 \| #241	0.511	0.614	Snapshot omitted
4	242	1	missense	SOX9	p.Y84H	0.015	0.000	4311.5	VLKGHDWTL	SQVLKGHDWTLVPMP	Tier 4 \| #242	0.519	0.607	Snapshot omitted
4	243	1	missense	MUC13	p.S118P	0.025	0.010	130.1	SLATPDIITA	TPDIITASSPNDGLI	Tier 4 \| #243	0.578	0.549	Snapshot omitted
4	244	1	missense	FAT1	p.G2653D	0.059	0.010	7296.8	LSDVITTKESL	LEINKLSDVITTKES	Tier 4 \| #244	0.591	0.533	Snapshot omitted
4	245	1	missense	ATXN2	p.A1078T	0.016	0.010	134.1	HPSHVQPTY	VFTIHPSHVQPTYTN	Tier 4 \| #245	0.391	0.693	Snapshot omitted
4	246	1	splice	PIK3CB	p.Q207H	0.019		1001.7	FENCHDVFSF	GGKLIVAVHFENCHD	Tier 4 \| #246	0.443	0.658	Snapshot omitted
4	247	1	splice	B4GALT5	p.L265R	0.034		15.3	KLDKYMYRL	DKYMYRLPYTEFFGG	Tier 4 \| #247	0.697	0.378	Snapshot omitted
4	248	1	missense	DNM2	p.F258Y	0.015		216.3	LAAERKYFL	GKKDIRAALAAERKY	Tier 4 \| #248	0.548	0.571	Snapshot omitted
4	249	1	missense	ZMYND8	p.G533D	0.018	0.010	124.6	TTDSILNL	TDSILNLNLDRSKAE	Tier 4 \| #249	0.663	0.423	Snapshot omitted
4	250	1	missense	OSBPL7	p.L306R	0.022		22.6	RAQKVHSSL	PPTDYAHLQRSFWAR	Tier 4 \| #250	0.585	0.523	Snapshot omitted

Cancer VaccineTarget Report

Joint Immunogenicity Scatter

Top-30 Vaccine Candidates

Cancer Vaccine
Target Report